新型转录共激活因子人类p100蛋白的抗体制备  

Preparation of Specific Antibody Against Human P100 Protein-A Noval Transcription Co-activator

作　　者：东莉洁[1] 邢冬红[1] 白虹[1] 步天栩[1] 姚智[1] 杨洁[1] 

机构地区：[1]天津医科大学免疫学教研室,300070

出　　处：《天津医药》2006年第11期786-788,共3页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30300070);高等学校博士学科点专项科研基金资助项目(项目编号:20040062003);天津市应用基础研究重点项目(项目编号:043802811);国家教育部新世纪人才支持计划资助项目(项目编号:NCET-04-0245)

摘　　要：目的:制备抗p100蛋白的特异性抗体。方法:利用谷胱甘肽-S-转移酶(GST)融合蛋白的方法纯化人类p100蛋白Tudor片段作为抗原,经免疫动物获得多克隆抗血清,并利用Westernblot的方法检测抗体的特异性。结果:获得的抗p100蛋白抗体,经体内和体外试验验证的确可与其相应抗原特异性结合。结论:制备的抗p100蛋白的抗体通过与其抗原即人类p100蛋白在体内和体外试验中的特异性结合,为进一步探讨生理条件下p100蛋白在细胞内信号传导通路的作用提供了可靠工具。Objective： To set up a system to generate the specific antibody against human plO0 protein. Methods： To generate polyclonal, antibody GST fusion protein containing p100 TD domain was purified as antigen to immunize rabbits. Western blot was performed to identify the specifity of the antibody. Results： The antibody against human p100 protein was capable of interacting with its corresponding antigen both in vivo and in vitro in this study. Conclusion： The interaction between human plO0 protein and its antibody was highly specific, therefore, a reliable and convenient tool is offered for further studying the role of p100 in signal transduction in vivo under physiological condition.

关 键 词：DNA结合蛋白质类 免疫球蛋白个体基因型 抗体特异性 

分 类 号：R770.43[医药卫生—眼科]