TEIF蛋白在软组织肉瘤中的表达及意义  

Expression of TEIF protein in soft tissue tumors and its significance

作　　者：龚一蕾[1] 李挺[2] 郭华[2] 孙颖[1] 迟英凯[1] 凌云[3] 沈琪[1] 刘海静[1] 侯琳[1] 张波[1] 

机构地区：[1]北京大学医学部病理学系,100083 [2]北京大学第一医院病理科 [3]齐齐哈尔医学院病理教研室

出　　处：《中华病理学杂志》2006年第11期651-655,共5页Chinese Journal of Pathology

基　　金：国家自然科学基金资助项目(30570691);教育部博士点基金资助项目(2000147)

摘　　要：目的观察TEIF蛋白在软组织肉瘤组织中的表达及与类型、分级的关系。方法通过原核表达TEIF蛋白免疫制备多克隆抗体,免疫印迹鉴定。以组织芯片-免疫组织化学(EnVision法)检测166例软组织肉瘤和28例良性肿瘤及瘤样病变标本TEIF蛋白表达。结果制备多克隆抗体经Western印迹鉴定与TEIF具有特异结合性。免疫组织化学显示166例软组织肉瘤中TEIF蛋白阳性97例,阳性率为58%(97/166),高于良性肿瘤及瘤样病变(11%,3/28)(P<0.05)。阳性主要分布为滑膜肉瘤94%(16/17)、原始神经外胚叶瘤(PNET)91%(21/23),两者高于隆突性皮肤纤维肉瘤43%(6/14)、黏液性纤维肉瘤38%(6/16)、恶性外周神经鞘膜瘤36%(8/22)、脂肪肉瘤32%(6/19)(P<0.05),而与恶性纤维组织细胞瘤75%(15/20)、横纹肌肉瘤(7/10)、平滑肌肉瘤64%(9/14)差异无统计学意义(P>0.05)。同时,TEIF蛋白的弥漫阳性表达(≥2+)多存在于PNET和滑膜肉瘤,分别为83%(19/23)和76%(13/17)。在法国癌症中心联盟(FNCLCC)分级中,19例Ⅰ级肉瘤中TEIF阳性表达为32%(6/19),44例Ⅱ级肉瘤阳性率为48%(21/44),两者中2+以上阳性分别为11%(2/19)、32%(14/44),而70例Ⅲ级肉瘤中TEIF阳性表达为84%(59/70)和2+以上阳性为70%(49/70)。Ⅲ级组的TEIF阳性率(及2+以上阳性率)显著高于Ⅱ级组和Ⅰ级组(均为P<0.05),而后两者间则差异无统计学意义(P>0.05)。结论TEIF蛋白表达存在于软组织肉瘤,尤其高表达于PNET、滑膜肉瘤,并与肉瘤的组织学分级有关。Objective To evaluate the expression of TEIF protein in human tumors of soft tissue and its signifieanee. Methods Anti-TEIF polyelonal antibody was generated by immunization of E. eoli expressed His-TEIF protein. The expression of TEIF in 166 eases of sareomas and 28 ease benign tumors or tumor-like lesions of soft tissue arranged in tissue ehip was analyzed by immunohistoehemistry. Results Polyelonal antibody obtained from immunized rabbit was verified in Western blot to prove its speeifie reaetivity with native TEIF protein. The immunohistoehemieal staining of TEIF showed that about 58% （97/ 166） of sareomas were positive and signifieantly different from that of benign tumors or tumor-like lesions （11%, 3/28）. The positive staining was predominantly in synovial sareoma 94% （16/17）, primitive neuroeetodermal tumor （PNET） 91% （ 21/23 ） , both of which were signifieantly higher than 43 % （ 6/14 ） of dermatofibrosareoma protuberans, 38% （6/16） of myxofibrosareoma, 36% （ 8/22 ） of malignant peripheral nerve sheath tumor, 32% （ 6/19 ） of liposareoma, （ P 〈 0. 05, respeetively）, but not higher than 75 % （ 15/ 20） of malignant fibrous histioeytoma, 70% （7/10） of rhabdomyosareoma or 64% （9/14） of leiomyosareoma. Meanwhile, strong positive staining of TEIF （ ≥ 2 ＋ ） was frequently observed in PNET （83% , 19/23） and synovial sareoma （76% , 13/17）. With respeet to FNCLCC grading, 19 eases of grade Ⅰ sareoma TEIF was 32% （6/19） and strong positive was 11% （2/19） ,44 eases of grade Ⅱ sareoma was 48% （21/44） and 32% （ 14/44）, and 70 of grade Ⅲ was 84% （59/70） and 70% （49/70）. The rate of either positive or strong positive in grade Ⅲ sarcoma was significantly different from that of either grade Ⅰ or Ⅱ （ P 〈 0. 05 ）, but no differenee between the latter two groups （ P 〉 0. 05 ）. Conclusions TEIF protein could be detected in large part of soft tissue sarcomas, and it not only over-expressed in most of PNET, syno

关 键 词：软组织肿瘤 基因表达 组织芯片 免疫组织化学 

分 类 号：R738.6[医药卫生—肿瘤]