血浆高密度脂蛋白对内皮祖细胞生长特性的影响  

The effect of serum high-density lipoprotein on the growth rate of human endothelial progenitor cells

作　　者：张秋华[1] 尹洪超[1] 张俊华[2] 刘佩毛[1] 张华[1] 佘铭鹏[1] 

机构地区：[1]中国医学科学院基础医学研究所中国协和医科大学基础医学院病理学系,北京100005 [2]中国医学科学院中国协和医科大学北京协和医院麻醉科

出　　处：《中华病理学杂志》2006年第11期672-676,共5页Chinese Journal of Pathology

基　　金：国家自然科学基金资助项目(30570720)

摘　　要：目的研究血浆高密度脂蛋白(HDL)对脐血内皮祖细胞(EPC)生物学特性的影响。方法应用密度梯度离心法从人脐带血中分离单个核细胞,接种于M200培养液。经血管内皮生长因子(VEGF)诱导后,进行细胞特异性标志物CD133、CD34、血管内皮细胞生长因子受体2和第八因子相关抗原检测,鉴定其分化为内皮祖细胞。在细胞分化的特定阶段,通过MTT、共聚焦显微镜检查和流式细胞术等方法,检测HDL对EPC增殖、抗原表达及细胞周期的影响。结果在细胞分化阶段,HDL明显促进内皮祖细胞增殖,促使细胞由G1期向S期的转化。细胞增殖指数增加15.3%。cyclinD1表达增加89.9%。结论脐带血中含有内皮祖细胞,在一定条件下可分化为内皮细胞。HDL对内皮祖细胞在体外的分化、增殖有重要的调节作用。这为动脉粥样硬化的发病机制研究和防治提供了一个新思路。Objective To study the effect of high-density lipoprotein （HDL） on the proliferation of endothelial progenitor cells （EPC） isolated from human umbilical cord blood; to further explore its effect on prevention and development of atherogenesis. Methods EPC isolated by density gradient centrifugation were cultured in a M200 medium. Immunofluorescence staining for CD133 ,CD34, KDR and Factor Ⅷ were adopted respectively as the specific markers for identification. The effect of HDL on EPC proliferation was estimated on the 7th day of cell cultivation using MTF assay, confocal microscopy and fluorescence activated cell sorting. Results HDL, when incubated with EPC, was able to promote remarkably the proliferation rate of EPC, dose- and time- dependent. HDL participated in the transcriptional regulation of cell cycle by affecting the regulatory proteins such as cyclin D1. Conclusions A subtype of progenitor cells was isolated from human cord blood with a potential of differentiating into mature endothelial cells （ known as endothelial progenitor cells）. HDL plays an important role on EPC fluorescence activated cell sorting differentiation and proliferation. Further studies are required to identify the signal pathway and the molecular mechanism of HDL effect on EPC proliferation.

关 键 词：脂蛋白类 HDL 内皮 血管 干细胞 

分 类 号：R361[医药卫生—病理学]