慢性肝病患者血清基质金属蛋白酶-2及其抑制物的变化和临床意义  

The changes and significance of serum MMP-2 and TIMP-2 levels in patients with Chronic Hepatitis B and Liver Cirrhosis

作　　者：何建方[1] 刘江[1] 钱福初[1] 戴利成[1] 施柏年[1] 周建方[1] 

机构地区：[1]湖州市中心医院中心实验室,浙江湖州313000

出　　处：《中国实验诊断学》2006年第11期1277-1279,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨慢性乙型肝炎(CHB)和乙型肝炎后肝硬化患者血清中的基质金属蛋白酶(MMP-2)、基质金属蛋白酶组织抑制因子(TIMP-2)的变化及其临床意义。方法用ELISA法检测75例CHB患者、41例乙型肝炎后肝硬化患者和30例健康献血员血清MMP-2和TIMP-2含量,用放射免疫法检测透明质酸(HA)、层粘连蛋白(LN)和Ⅳ型胶原(CⅣ)的含量,分析MMP-2、TIMP-2与肝纤维化指标之间的相关性。结果CHB中度、CHB重度、肝硬化A级、B级、C级患者血清MMP-2均明显高于正常对照组(P<0.01),且肝硬化C级>肝硬化B级>肝硬化A级>CHB重度>CHB中度>CHB轻度(P<0.05,0.05,0.05,0.01,0.01),与HA、LN、CⅣ呈显著性正相关(r=0.913,0.845,0.897。P均<0.01);CHB重度、肝硬化A级、B级、C级患者血清TIMP-2均明显高于正常对照组(P<0.01),肝硬化C级与CHB轻度、CHB中度、CHB重度比有显著性差异(P<0.01,0.01,0.05),肝硬化B级、肝硬化A级、CHB重度与CHB轻度、CHB中度比有显著性差异(P<0.01),与HA、LN、CⅣ呈显著性正相关(r=0.693,0.574,0.646。P均<0.01)。结论血清MMP-2、TIMP-2在慢性肝病患者肝纤维化过程中明显升高,可做为判断肝纤维化程度的指标,尤其以MMP-2的诊断意义更大。Objectlve To investigate the changes and significance of serum matrix metalloproteinase-2（ MMP-2 ）and issue inhibitor of metalloprotienase-2（TIMP-2） in patients with chronic hepatitis B（CHB） and liver cirhosis（LC） .Methods Serum MMP-2 and TIMP-2 were detected by ELISA and hepatic fibrosis index： hyaluronate（HA）, laminin（LN） and Ⅵ Collagen（ⅥC） were detected by RIA in 75 patients with CHB , 41 patients with LC and 30 healthy persons, then analyze their relationship. Results The serum MMP-2 concentrations in patients with mild ,moderate, sever degree CHB patients and LC Child-pugh＇s group A, B and C patients were significantly highter than those in normal control, And there was significant differences in the serum MMP-2 levels among these six groups patients respectively. The serum MMP-2 levels were positively realated to the levels of HA, LN, C Ⅳ, respectively;The serum TIMP-2 concentrations in patients with sever degree CHB patients and LC Child-pugh＇ s group A, B and C patients wre significantly highter than those in normal control, Significant differences were seen between the serum TIMP-2 levels of LC Child-pugh＇ s group C and there groups CHB patients, and between LC Child-pugh＇ s group A, B, sever degree CHB patients and mild, moderate degree CHB patients, respectively. The sertun TIMP-2 levels were positively realated to the levels of HA, LN, C Ⅳ, respectively. Conclusion Since the serum levels of MMP-2 and TIMP-2 was significandy ineeased in CHB and LC,measurement of them can be used as diagnostic indices for liver fibrosis in chronic liver disease,and senml MMP- 2 level is more useful.

关 键 词：基质金属蛋白酶-2 基质金属蛋白酶-2组织抑制因子 慢性乙型肝炎 肝硬化 

分 类 号：R575[医药卫生—消化系统]