牛至超临界CO_2萃取物的GC指纹图谱  

Fingerprint Analysis of Supercritical Fluid CO_2 Extract of Origanum vulgare L.by GC

作　　者：王光忠[1] 孙丽娟[1] 刘红兵[1] 许汉林[1] 刘焱文[1] 

机构地区：[1]湖北中医学院中药化学与中药资源重点实验室,武汉430061

出　　处：《中国天然药物》2006年第6期452-455,共4页

基　　金：国家自然科学基金资助项目(No.30370148)~~

摘　　要：目的:采用GC法测定牛至CO2萃取物的指纹图谱。方法:采用超临界CO2萃取技术提取牛至挥发油。GC色谱条件:DB-17石英毛细管柱(30m×0.25mm,膜厚0.25μm),载气为氮气,流速1.5mL.min-1,分流比201,程序升温:80℃(2min)4℃/min270℃(2min)。结果:指纹图谱中标示了17个共有峰并采用GC-MS进行了鉴别,其峰面积之和大于总峰面积的90%,方法精密度、稳定性、重现性均符合指纹图谱有关规定。结论:本方法为牛至药材的质量控制提供了依据。AIM： To develop the chromatographic fingerprint of supercritical fluid CO2 extract of Origanum vulgate L. by GC. METHOD： Supereritical fluid CO2 extraction was used to extract the essential oil from Origanum vilgare L.. The chromatographic conditions were as follows： DB-17（30 m×0.25 mm,0.25μm）capillary column; carrier gas N2, flow rate 1.5 mL·min^-1, split ratio 20 1 ; column temperature program 80 ℃ lasting 2min,from 80 ℃to 270 ℃ at rising rate 4 ℃/min and keeping 2min. RESULT： 17 peaks were identified by GC-MS. The peak area of these components was over 90% of total area in the chromatographic fingerprint. RSD of precision and reproducibity was in the range of 5%. CONCLUSION： The method may provide reliable foundation of quality control of Origanum vulgate L. .

关 键 词：牛至 超临界CO2萃取 GC 指纹图谱 

分 类 号：TQ460.72[医药卫生—药物分析学]