鱼油对大鼠心肌细胞膜上β-受体和腺苷酸环化酶活性的影响  

EFFECT OF FISH OIL ON B-ADRENOCEPTORS AND THE ACTIVITY OF ADENYLATE CYCLASE ON MYOCARDIAL MEMBRANE IN RATS

作　　者：周建政[1] 吴葆杰[1] 张岫美[1] 

机构地区：[1]山东医科大学基础部药理学教研室

出　　处：《中国海洋药物》1996年第3期5-9,共5页Chinese Journal of Marine Drugs

摘　　要：探讨了鱼油对大鼠心肌细胞膜上β-受体和腺苷酸环化酶(adenylate cyclase,AC)活性的影响。鱼油1.4ml/(kg·d)ig 2w对大鼠心肌细胞膜上AC的基础活性无显著影响,但可显著地抑制异丙肾上腺素(ISO)激活的AC活性,放射配体饱和实验结果显示,同正常对照相比,鱼油的摄入对B_(max)和K_d无显著影响,但同羊油组相比,可显著地降低β-受体与其配体的亲和力。同正常对照相比,羊油的摄入一方面可显著地升高β-受体与其配体和亲和力,另一方面又可显著地降低β-受体的数目。结果提示,鱼油可能主要影响AC的激活而不是主要影响β-受体本身的功能。Effects of fish oil on B-adrenoceptors as well as the activity of adenylate cyclase (AC) on rat myocardial membrane were investigated. Supplementation with fish oil had no significant effect on basal activity of AC on myocardial membrane, whereas it could markedly inhibit the AC activity stimulated by isoproterenol (ISO). Radioligand binding assays showed that supplementation with fish oil had no effect on Bmax, and Kd, compared with saline control. However, supplementation with sheep oil could markedly reduce both the Ka and Bmax, compared with saline control. And the Ka value of sheep oil group was greatly decreased than that of fish oil group. The results suggested that supplementation with fish oil affected mainly on the activation of AC and not on B-adrenoceptor itself.

关 键 词：鱼油 腺苷酸环化酶 活性 Β受体 心肌 烯酸 

分 类 号：R977.6[医药卫生—药品] R965.1[医药卫生—药学]