大鼠FEZ1基因的克隆及其在中枢神经系统的表达  

CLONING AND EXPRESSION OF RAT FEZ1 GENE IN THE CENTRAL NERVOUS SYSTEM

作　　者：何江虹[1] 朱婷[1] 夏春林[2] 张万钰[1] 张晔[2] 万明辉[2] 

机构地区：[1]南通大学江苏省神经再生重点实验室,南通226001 [2]苏州大学医学院细胞神经生物学研究室,苏州215123

出　　处：《神经解剖学杂志》2006年第6期653-656,共4页Chinese Journal of Neuroanatomy

基　　金：南通市青年学术带头人带课题进修计划项目(Z5007);江苏省高校自然科学研究计划(05KJB310121);江苏省分子神经生物学重点实验室项目(KJS02050)资助项目

摘　　要：轴突成束和延伸蛋白ζ-1(fasciculationandelongationproteinzeta-1,FEZ1),是线虫UNC-76蛋白在哺乳动物中的一种同系物,与轴突向外生长、成束、延伸相关。为了克隆该蛋白的基因并观察其在中枢神经系统(CNS)的表达情况,本研究通过FEZ1的特异引物从SD大鼠脑组织中经逆转录-聚合酶链反应(RT-PCR)获得FEZ1基因;通过RT-PCR法分析FEZ1在生后7d的SD大鼠CNS的不同部位(大脑皮层、嗅球、脊髓)的表达情况。成功获得FEZ1的基因克隆,并观察到生后7d的SD大鼠的大脑皮层、嗅球、脊髓内均有FEZ1表达,且脊髓的表达水平较高。本研究结果为进一步研究大鼠FEZ1基因的生物学活性及在神经系统内的表达和应用奠定了基础。Fasciculation and elongation protein zeta-1 （ FEZ1 ） is a mammalian homologue of the Caenorhabditis elegans UNC-76 protein, which is involved in axonal outgrowth, fasciculation and elongation. To clone FEZ1 gene and study the expression of FEZ1 in the central nervous system（CNS） , the rat FEZ1 cDNA was prepared from the brain of rat total RNA extracts by reverse transcription-polymerase chain reaction with special primers for FEZ1. The expression of FEZ1 was detected in the different regions （ cerebral cortex, olfactory bulb and spinal cord） of the CNS of postnatal 7 day （P7） rats. The rat FEZ1 cDNA was successfully obtained and FEZ1 was observed to be expressed in the cerebral cortex, olfactory bulb and spinal cord of the CNS on P7 and there was a higher expression of FEZ1 in the spinal cord. Therefore, the present results provided a base for the future study on biological function, its expression and application of the FEZ1 in the nervous system.

关 键 词：FEZ1 基因克隆 表达 中枢神经系统 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]