缺氧对大鼠肝细胞转化生长因子β_1表达的影响  

Effects of anoxia on expression of transforming growth factor-β_1 in rat liver

作　　者：李侠[1] 詹皓[1] 齐颖[1] 王琼[1] 赵安东[1] 王喆[2] 笪冀平[2] 施新猷[3] 

机构地区：[1]中国人民解放军空军航空医学研究所,北京市100036 [2]中国人民解放军空军总医院病理科,北京市100036 [3]第四军医大学动物实验室,陕西省西安市710032

出　　处：《世界华人消化杂志》2006年第33期3180-3183,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨缺氧对转化生长因子β1(transform-inggrowthfactor-β1,TGF-β1)在肝细胞表达的影响.方法:选择SD大鼠20只,采用气管阻塞的方法建立轻、中、重度的缺氧模型,15min和60min采集肝脏标本,用免疫组化技术SABC法检测肝细胞转化生长因子β1表达的变化.结果:在正常肝脏中TGF-β1极少表达或无表达(15和60min分别为3.6±1.9,3.6±1.9),随着缺氧时间的延长与缺氧程度的加重,肝细胞转化生长因子β1表达逐渐增加(轻度缺氧15和60min分别为8.5±2.0,15.2±3.1;中度缺氧15和60min分别为18.4±3.5,63.7±3.8;重度缺氧15和60min分别为68.9±3.2,86.7±3.6).结论:大鼠缺氧条件下肝脏TGF-β1表达增加可促进细胞外基质的合成,有利于肝细胞的再生与修复.AIM： To investigate the effect of anoxia on the expression of transforming growth factor-β1 （TGF-β1） in the rat liver. METHODS： A total of 20 Sprague Dawley rats were used in this study. Trachea obstructive method was employed to establish the animal model of mild, moderate, and severe anoxia. Liver samples were collected 15 and 60 min after obstruction for the examination of TGF-β1 expression using immunohistochemical technique. RESULTS： TGF-β1 was not or lowly expressed in normal rats （3.6 ± 1.9 and 3.6 ± 1.9, respectively at 15 and 60 min）. The level of hepatic TGF-β1 expression was increased with prolonging of anoxia time and enhancement of anoxia degree. The expression of hepatic TGF-β1 at 15 and 60 min was 8.5 ± 2.0 and 15.2 ± 3.1 under mild anoxia, 18.4 ± 3.5 and 63.7 ± 3.8 under moderate anoxia, and 68.9 ± 3.2 and 86.7 ± 3.6 under severe anoxia, respectively. There were significant differences between normal and anoxia rats （P 〈 0.05 or P 〈 0.01）. CONCLUSION： TGF-β1 expression is increased under the condition of anoxia, which accelerates the formation of extracellular matrix, and further contributes to the repair and regeneration of liver cells.

关 键 词：缺氧 转化生长因子Β1 肝细胞 大鼠 免疫组化 

分 类 号：R575[医药卫生—消化系统]