实验性急性肝内胆汁淤积幼年大鼠胃肠消化间期移行性肌电复合波的变化  

Changes of gastrointestinal interdigestive migrating myoelectric complex in rat intrahepatic cholestasis induced by alpha-naphthyl-isothiocyanate

作　　者：胡玉莲[1] 王晓东[1] 黄志华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,湖北省武汉市430030

出　　处：《世界华人消化杂志》2006年第32期3098-3102,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨异硫氰酸萘酯(ANIT)所致刚断乳大鼠急性肝内胆汁淤积胃肠消化间期移行性肌电复合波(MMC)的变化.?方法:?56只刚断乳SD大鼠被随机分为正常对照组(n?=?16)、中毒组(n?=?40).?两组分别随机取8只在胃窦、十二指肠、空肠分别慢性埋置三对银丝电极;?其余大鼠同时行假手术.?术后7-10?d,?埋置电极组大鼠均在清醒、空腹、自由活动状态下记录胃肠道MMC.?中毒组按200?mg/kg一次性灌服ANIT诱发大鼠急性肝内胆汁淤积病变,?观察灌服ANIT后中毒组在48,?96,?144,?192?h胆汁流量、血中TB和ALT值及胃肠MMC的变化.?结果:中毒组灌服ANIT后,?48?h时胆汁流量中断,?血清TB和ALT明显升高;?48?h后其胆汁流量逐渐增加,?血清TB和ALT逐渐下降,?于192?h基本恢复.?在ANIT灌胃前,?埋置电极的所有大鼠在清醒、禁食状态下均记录到典型的MMC节律性电活动,?正常对照组与中毒组之间差异无显著性意义;?中毒组灌服ANIT后,?MMC节律完全消失,?代之以Ⅱ期样节律紊乱波;?144?h后,?中毒组MMC节律运动开始恢复;?192?h时中毒组MMC均为节律运动,?但中毒组MMC持续时间(911.67±140.47?s)较正常对照组(682.87±77.39?s)明显延长,?其中主要是Ⅱ期持续时间延长(414.12±69.21?vs?150.28±35.45?s),?而Ⅲ期持续时间略缩短(121.21±27.38?s?vs?170.27±38.98?s)?,?差异有显著意义(P<0.05).结论:?急性肝内胆汁淤积时胃肠MMC表现MMC节律短暂消失或MMC周期延长;?其部分原因可能与消化间期胆汁流量减少有关.AIM： To investigate the changes of gastrointestinal interdigestive migrating myoelectric complex （MMC） in the weaned rat models of acute intrahepatic cholestasis induced by alphanaphthyl-isothiocyanate （ANIT）. METHODS： A total of 56 weaned Sprague Dawley rats were randomly divided into two groups： control group （n = 16） and toxication group （n = 40）. Three pairs of bipolar silver electrodes were chronically implanted in the antrum, duodenum and jejunum of 8 rats from each group in random. Sham operation was performed in the other rats of both groups. Seven to ten days after the operation, MMC was recorded in all the rats with implanted electrodes. Then, all of the rats in toxication group received a single intragastric administration of ANIT （200 mg/kg） in order to induce acute intrahepatic cholestasis. The gastrointestinal MMC was recorded in conscious and fasting status 48, 96, 144, 192 h after ANIT was given. The levels of biliary flow, total bilirubin （TB） and alanine aminotransferase （ALT） were recorded at the same time. RESULTS： Forty-eight hours after ANIT was given, the biliary flow of rats in toxication group was almost ceased completely while the levels of ALT and TB were increased remarkably. Then, they were all improved gradually till the 192th hour. A typical MMC pattern was seen in all the normal conscious and fasting rats, and disappeared temporarily after ANIT was given. One hundred and forty-four hours later, the MMC pattern began to restore gradually and recovered at the 192th hour. However, the prolonged cycle duration of MMC （911.67 ± 140.47 s vs 682.87 ± 77.39 s, P 〈 0.05） was observed in the toxication group at the 192th hour and characterized by an increased duration of phase Ⅱ-like activity （414.12 ± 69.21 s vs 150.28 ± 35.45 s, P 〈 0.05） and decreased duration of phase Ⅲ activity （121.21 ± 27.38 s vs 170.27 ± 38.98 s, P 〈 0.05）. CONCLUSION： MMC by a prolonged MMC changes in the weaned disappearance followed is the pat

关 键 词：肝内胆汁淤积 消化间期 移行性肌电复合波 胆汁流量 

分 类 号：R57[医药卫生—消化系统]