双歧杆菌在5-氟尿嘧啶作用下的适应性变化  

Adaptive changes of bifidobacteria in the presence of 5-fluorouracil

作　　者：刘俊康[1] 邓渝[1] 吴小兰[1] 魏海龙[1] 徐启旺[1] 

机构地区：[1]第三军医大学医学检验系生物波研究室,重庆市400038

出　　处：《世界华人消化杂志》2006年第32期3139-3142,共4页World Chinese Journal of Digestology

基　　金：重庆市科委自然科学基金计划资助项目;No.CSTC2006BB5068

摘　　要：目的:研究双歧杆菌在5-氟尿嘧啶(5-fluorou-racil,5-FU)作用下的适应性变化特点.方法:采用药敏实验、染色形态观察、原子力显微镜扫描观察以及耐药菌株生长实验鉴定等方法观察双歧杆菌的生长情况及形态特征.结果:在5-FU作用下,双歧杆菌菌体加长,断裂减少,转变成丝状体,长度在10μm以上,两端呈分枝状,相对高度约1μm,并且在抑菌环内还产生了耐药菌株.这些丝状体在正常的生长条件下又可以恢复正常生长.结论:化疗药物可以直接影响双歧杆菌的生长,而双歧杆菌对化疗药的作用可产生显著的适应性变化.AIM： To observe the characteristics for the adaptive changes of bifidobacteria under the influence of 5-fluorouracil （5-FU）. METHODS： The growth and morphological characteristics of bifidobacteria in the presence of 5-FU were acquired by drug-sensitivity test, gram stain and atomic force microscopic scan- ning as well as the identification of the 5-FU- resitant isolates. RESULTS： In the presence of 5-FU, bifidobacteria were lengthened with fewer ruptures, becoming filament-like, with a length of more than 10 μm. The bifidobacteria were ramose at both ends and the relative height was about 1 μm. The 5-FU-resistent bifidobacteria were also found within the inhibition zone. Given the normal growth conditions, the filament-like bifidobacteria restored to the normal growth again. CONCLUSION： 5-FU can directly influence the growth of bifidobacteria, whereas bifidobacteria exhibit significant adaptive changes to the action of 5-FU.

关 键 词：双歧杆菌 化疗药物 5-氟尿嘧啶 生物波 原子力显微镜 

分 类 号：R57[医药卫生—消化系统]