Cysteinyl leukotriene receptors CysLT_1 and CysLT_2 are upregulated in acute neuronal injury after focal cerebral ischemia in mice  

作　　者：Yan-jun ZHANG Lei ZHANG Yi-lu YE San-hua FANG Yu ZHOU Wei-ping ZHANG Yun-bi LU Er-qing WEI 

机构地区：[1]Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310058 [2]Zhejiang Provincial Centre for Disease Control and Prevention, Hangzhou 310009, China

出　　处：《Acta Pharmacologica Sinica》2006年第12期1553-1560,共8页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China(№30271498).

摘　　要：Aim： To determine whether cysteinyl leukotriene receptors （CysLT1 and CysLT2） are upregulated in acute neuronal injury after focal cerebral ischemia in mice, and to confirm CysLT1 receptor localization. Methods： After permanent focal cerebral ischemia was induced by middle cerebral artery occlusion （MCAO）, neurological deficits and neuron loss were determined at various time points within 48 h. The mRNA expressions of CysLT1 and CysLT2 receptors in the brain were analyzed by RT-PCR. CysLT1 receptor localization was detected by double immunofluorescence. Results： Neurological deficits and neuron loss were found 6, 24 and 48 h after MCAO. The mRNA expressions of both CysLT1 and CysLT2 receptors were upregulated in the ischemic hemisphere 1, 24, and 48 h after MCAO with peaks at 24 h. The CysLT1 receptor was selectively localized in neurons 24 h after MCAO. Conclusion： CysLT1 and CysLT2 receptors are upregulated in acute neuronal injury after focal cerebral ischemia, and the CysLT2 receptor is localized in neurons after ischemia.Aim： To determine whether cysteinyl leukotriene receptors （CysLT1 and CysLT2） are upregulated in acute neuronal injury after focal cerebral ischemia in mice, and to confirm CysLT1 receptor localization. Methods： After permanent focal cerebral ischemia was induced by middle cerebral artery occlusion （MCAO）, neurological deficits and neuron loss were determined at various time points within 48 h. The mRNA expressions of CysLT1 and CysLT2 receptors in the brain were analyzed by RT-PCR. CysLT1 receptor localization was detected by double immunofluorescence. Results： Neurological deficits and neuron loss were found 6, 24 and 48 h after MCAO. The mRNA expressions of both CysLT1 and CysLT2 receptors were upregulated in the ischemic hemisphere 1, 24, and 48 h after MCAO with peaks at 24 h. The CysLT1 receptor was selectively localized in neurons 24 h after MCAO. Conclusion： CysLT1 and CysLT2 receptors are upregulated in acute neuronal injury after focal cerebral ischemia, and the CysLT2 receptor is localized in neurons after ischemia.

关 键 词：cysteinyl leukotriene receptor cerebral ischemia neuronal injury mRNA expression 

分 类 号：R74[医药卫生—神经病学与精神病学]