胃癌高发区IL-1基因多态性、H.pylori感染和胃黏膜萎缩关系的研究  

The relation between H. pylori infection,Interleukin-1B gene polymorphism and musolar atrophy in high prevalence region of gastric cancer

作　　者：江绍伟[1] 徐展[1] 高尚民[1] 

机构地区：[1]湖北中医学院医学检验与技术系,湖北武汉430064

出　　处：《现代肿瘤医学》2006年第12期1565-1567,共3页Journal of Modern Oncology

摘　　要：目的：分析胃癌高发区普通人群的IL-1B-511基因多态性、H．pylori感染率及胃粘膜萎缩的血清学指标（血清胃蛋白酶原Ⅰ（pepsinogen Ⅰ，PGI）、胃蛋白酶原Ⅰ／胃蛋白酶原Ⅱ的比值（PGⅠ／PGⅡ）和胃泌素（gastrin）浓度，探讨IL-1B-511基因多态性对幽门螺旋杆菌（Helicobacter pylori，Hp）感染后胃黏膜萎缩的影响。以期进一步明确IL-1B基因多态性与胃黏膜萎缩关系，IL-1B基因多态性增加胃癌发病危险性的可能机理。方法：采用PCR-限制性长度片段多态性（RFLP）分析法检测胃癌高发区陕西省普通人群169例的基因型。采用酶联免疫吸附法（ELISA）检测上述人群的Hp感染率、PGⅠ、PGⅡ和Gastrin的浓度。结果：在胃癌高发区，Hp阳性者PGI／PGII显著低于Hp阴性者（P〈0．01），但IL-1B-511T／T基因型人群PGI／PGII与IL-1-B-511C／T和C／C基因型者无显著差异（P值均〉0．05）。血清胃泌素浓度与IL-1B-511的基因型没有明确的关系（P〉0．05）。结论：在胃癌高发区，Hp感染可能在20～23岁年龄段已是胃黏膜萎缩的危险因素，IL-1B-511 T／T基因型者可能增加Hp后胃黏膜萎缩形成的危险性；胃黏膜萎缩的发生、发展除与Hp感染和遗传有关外，环境因素可能起相当重要的作用。Objective：To investigate the relation between interleukin -1B gene polymorphism, Hpinfeetion and musolar atrophy. Methods： IL - 1B gene polymorphism of eommon people in high prevalenee region of gastrie eaneer were analyzed by PCR - RFLP,the Hp infeetion rate, PG I/II ratio and gastrin were examined using ELISA or RIA. Results： People with Hp infeetion have higher PG I level, but lower PG I/PG II ratio, eompared with individuals without Hp infeetion. PG I was similar among eaeh genotype not only in IL - 1B -511 loeus, the gastrin level was similar among eaeh genotype in IL - 1B -511 loeus no matter whether Hp infected or not. Conclusion ： In high prevalenee region, of gasteie eancer IL- 1B -511 T/T genotype is a risk faetor for musolar atrophy after Hp infeetion. Environmental faetor is a more important faetor besiedes Hp infeetion and Heredity.

关 键 词：白细胞介素-1 多态性 幽门杆菌 胃黏膜 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]