EB病毒与P16基因蛋白在子宫颈鳞癌中的表达  

Expression of EBV and p16 proteins in cervical squamous cell carcinoma by tissue microarray technique

作　　者：刘秀华[1] 肖秀丽[1] 龙汉安[1] 张娟[1] 刘俊[1] 

机构地区：[1]泸州医学院附属医院病理科

出　　处：《临床肿瘤学杂志》2006年第11期818-821,共4页Chinese Clinical Oncology

摘　　要：目的:分析子宫颈EBV感染对抑癌基因P16蛋白表达的影响,探讨EBV的致癌机理。方法:采用组织芯片技术和免疫组织化学S-P法,检测13例正常宫颈、39例宫颈上皮内瘤变和28例宫颈鳞癌组织中EBV及P16蛋白的表达。结果:EBV阳性表达率在正常宫颈、宫颈上皮内瘤变、宫颈癌组分别为15%(2/13)、51%(20/39)和68%(19/28),癌及上皮内瘤变组均明显高于正常宫颈组(P<0·05)。P16在正常宫颈组织与上皮内瘤变及宫颈癌中的阳性表达率分别为7·7%(1/13)、64%(25/39)和79%(22/28),三组间差异有显著性(P<0·05)。EBV感染与非感染的宫颈癌组织中,P16的阳性表达率分别为79%和78%,两组间无显著性差异(P>0·05)。结论:EBV感染与宫颈癌的发病密切相关,但其致癌机制可能不是通过影响抑癌基因P16的表达而引起的。Objective： To study the expression of EBV and P16 anti-oncogene in cervical carcinoma and approach EBV＇s mechanism of action. Methods ： Using tissue microarray technique and S-P immunohistochemical technique, we examined the expression of EBV and p16 in 13 normal cervical tissues, 39 cervical intraepithelial neoplasm and 28 cervical squamous cell carcinoma. Results： Expression of EBV positive in normal cervical tissues, cervical intraepithelial neoplasm and cervical squamous cell were 15% （2/13） , 51% （20/39） and 68% （19/28）, respectively. Carcinoma group and cervical intraepithelial neoplasm group were higher markedly than normal cervical tissues group（P 〈 0. 05） . Expression of P16 protein positive in normal cervical tissues, cervical intraepithelial neoplasm and cervical squamous cell carcinoma were 7.7% （1/13） , 64% （25/39） and 79% （22/28） , respectively. Expression of P16 protein positive was 79% in the EBV-positive group and 78vA in the EBV-negative group. There were no difference markedly between them（ P 〉 0. 05 ）. Conclusion： EBV infection is close relative with cervical carcinoma, but its mechanism of action couldn＇t be the P16 anti-oncogene gene inactivation.

关 键 词：EBV P16 宫颈癌 免疫组化 组织芯片 

分 类 号：R730.49[医药卫生—肿瘤] R737.33[医药卫生—临床医学]