人乳头状瘤病毒感染型别与子宫颈上皮内瘤变的关系  

Study on the relationship between human papilloma virus types and intraepithelial cervical neoplasia

作　　者：李怀芳[1] 朱新贤[1] 王昕[2] 李莉[1] 

机构地区：[1]同济大学附属同济医院妇产科,上海200065 [2]同济大学附属东方医院妇产科

出　　处：《上海医学》2006年第11期775-777,共3页Shanghai Medical Journal

摘　　要：目的采用聚合酶链反应(PCR)-寡核苷酸微流芯片进行人乳头状瘤病毒(HPV)分型,并研究子宫颈上皮内瘤变(CIN)与HPV感染型别的关系。方法对335例临床病例进行筛查、细胞学分级并分组,活检标本通过寡核苷酸微流芯片上特异性探针进行HPV分型。结果335例患者中,HPV阳性率为22．1%。宫颈良性疾病组的HPV阳性率为10．9%,CINⅠ、CINⅡ、CINⅢ和宫颈鳞癌组分别为47．2%、66．7%、68．4%和75．0%,均显著高于宫颈良性疾病组(P值均＜0．05)。结论随着CIN级别的升高,高危HPV型感染比例增加,寡核苷酸微流芯片检测能区分多种HPV型别,可用于临床宫颈上皮内瘤变的HPV筛查。Objective To study the relationship between intraepithelial cervical neoplasia（CIN） and types of Human papilloma virus（HPV） infection. Methods 335 cases were collected and grouped by cervical cytologic analysis. The HPV of CIN lesions was typed by microfluid chip. Results Of the 335 patients, the total HPV positive rate was 22. 1% （74/335）. The prevalence of benign cervical squamous epithelium group was 10. 9% （28/ 256）. Out of 36 CIN Ⅰ lesions, 17 were HPV DNA positive（54 % ）. In CIN Ⅱ group, 66.7 % （10/15） was associated with HPV infection. 68.4% （13/19） of CIN Ⅲ lesions were positive for HPV whereas 75%（6/8） of cervical carcinoma showed HPV positive. They showed significant increases in prevalence as comparing with cervical aquamous epithelium group（P 〈 0.05）. Conclusion The percentage of HPV infection increases with increase of CIN grade, demonstrating that the Oligo DNA mierofluid chip assay can discriminate many types of HPV and can be applied for HPV screening of clinical CIN.

关 键 词：人乳头状瘤病毒 子宫颈上皮内瘤变 寡核苷酸微流芯片 

分 类 号：R737.33[医药卫生—肿瘤]