不同方法制备的海星皂甙细胞毒性评价  

Cell Toxicity of Two Saponins Extracted by Different Methods from Starfish ( Asterias amurensis)

作　　者：文震[1] 党志[2] 宗敏华[1] 赵金华 李靖 

机构地区：[1]华南理工大学生物科学与工程学院轻工技术与工程博士后流动站,广东广州510640 [2]华南理工大学环境科学与工程学院,广东广州510640 [3]深圳企业博士后工作站海王分站,广东深圳518054

出　　处：《海洋通报》2006年第6期26-29,46,共5页Marine Science Bulletin

基　　金：中国博士后科学基金(2005037584);广东省自然科学基金重点项目(036556)资助

摘　　要：考察超临界CO2萃取、乙醇萃取两种方法制备的南海产多棘海星皂甙的溶血过程,采用分光光度法测定溶血指数,结合红细胞形态血观察,评价其细胞毒性。结果显示,两种方法制备的海星皂甙都具有明显溶血作用,超临界CO2萃取、溶剂萃取的海星皂甙溶血指数分别为19231,12821,超临界CO2萃取的海星皂甙保持了更强的细胞毒性,这为海星皂甙制备工艺的选择,探索其物质基础提供了参考依据。According to the hemolytic process, the cell toxicity of two saponins extracted by supercritical CO2 and organic solvent from Asterias amurensis in the South China sea was evaluated. The result showed all the saponins prepared by different methods had hemolysis to the red blood cell. Hemolytic activity showed that these saponins destroyed the membrane of the red blood cell The hemolytic index of two saponins extracted by supercritical CO2 and organic solvent were 19231 and 12821 respectively. The result provides reference for the selection extraction technique and material basis for toxicity.

关 键 词：海星 皂甙 溶血 超临界 

分 类 号：Q178.53[生物学—水生生物学]