生防菌株哈茨木霉H-13固体发酵条件的研究  

Studies on the solid fermentation conditions for the biocontrol agents of Trichoderma harzianum strain H-13

作　　者：王永东[1] 蒋立科[1] 岳永德[1] 花日茂[1] 江丽[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036

出　　处：《浙江大学学报（农业与生命科学版）》2006年第6期645-650,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家863"计划资助项目(2003AA241171)

摘　　要：研究了生防菌株哈茨木霉H-13固体发酵的条件，探索了碳源、氮源、磷酸盐、固料比、接种量及发酵温度对产孢量的影响．通过单因素试验和正交试验确定固体发酵最佳条件为：麸皮、玉米粉按3：1比例配伍，固料比水为1：0．4（g ·mL^-1），蔗糖15％，蛋白胨1．5％，硝酸铵1％，磷酸二氢钾0．05％，NaH2PO4-Na2HPO4（0．5％～0．50％），接种量为9％（mL·g^-1）；发酵温度为24℃～22℃～26℃，在此条件下发酵10d，产孢量高达1．0×10^10个·g^-1以上．固体发酵生产木霉分生孢子，与液体相比具有制作简单、成本低廉、孢子含量高、易于保存和运输等特点．Optimal solid-state fermentation conditions for biocontrol agents of Trichoderma harzianum strain H-13 were studied, the influencing factors on the conidial concentration were investigated, including carbon, nitrogen, phosphate, the solid material to water, inoculums and the temperature of fermentation. By single factor and orthogonal experiments, the optimal conditions for solid-state fermentation were obtained as following： the proportion of bran to maize and solid material to water were 3：1 and 1 ： 0.4 （g.mL^-1） respectively, sucrose 1.5%, peptone 1.5%, ammuonium nitrate 1%, potassium dihydrogen phosphate 0.05 %, NaH2PO4-Na2HPO4 （ 0.5 %-0.50 % ）, inoculum 9 % （ mL·g^-1）, the temperature of fermentation among 24℃-22℃-26℃ .Under this optimal fermentation conditions, the spore number were up to 1.0×10^10·g^-1 after 10 days. It is indicated that compared with liquid fermentation, the solid fermentation producing conidiophores has the characteristic of simpler manufacture, less cost, higher conidiophores content and easier to be preserved and transport.

关 键 词：哈茨木霉 固体发酵 生防菌 

分 类 号：Q939.9[生物学—微生物学]