Capillary Electrophoresis for the Simultaneous Determination of Metals by Using Ethylenediamine Tetraacetic Acid as Complexing Agent and Vancomycin as Complex Selector  

作　　者：THREEPROM Jirasak SOM-AUM Waraporn 林金明 

机构地区：[1]The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology Department of Chemistry, Tsinghua University, Beijing 100084, China [2]Department of Chemistry, Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand

出　　处：《Chinese Journal of Chemistry》2006年第12期1747-1753,共7页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （Nos. 20437020 and 20575008） and the Post Doctoral Research Scholarship of Department of Chemistry, Tsinghua University.

摘　　要：A new separation system of capillary electrophoresis for the simultaneous determination of metals by using ethylenediamine tetraacetic acid （EDTA） as complexing agent and employing vancomycin as complex selector was described. The Z-shape cell capillary electrophoresis was used to enhance the sensitivity for the determination of the complexes of Cu（Ⅱ）, Ni（Ⅱ）, Co（Ⅱ） and Fe（Ⅲ） with EDTA. The partial filling method （co-current mode） was used in order to increase the selectivity of the electrophoretic method, meanwhile vancomycin was not present at the detector path during the detection of metal-EDTA complexes. The vancomycin concentration, phosphate concentration and pH of the buffer strongly influenced mobility, resolution and selectivity of the studied analytes. Under the optimal condition, the relative standard deviations （n=5） of the migration time and the peak area were less than 3.14% and 7.35%, respectively. Application of the Z-shape cell capillary electrophoresis method with UV detection and vancomycin loading led to the reliable determination of these metal ions in tap water and the recoveries were 97%-101%. The detection limits based on a signal to noise ratio of 3 ： 1 were found in the range of 2-10 μg·L^-1.A new separation system of capillary electrophoresis for the simultaneous determination of metals by using ethylenediamine tetraacetic acid （EDTA） as complexing agent and employing vancomycin as complex selector was described. The Z-shape cell capillary electrophoresis was used to enhance the sensitivity for the determination of the complexes of Cu（Ⅱ）, Ni（Ⅱ）, Co（Ⅱ） and Fe（Ⅲ） with EDTA. The partial filling method （co-current mode） was used in order to increase the selectivity of the electrophoretic method, meanwhile vancomycin was not present at the detector path during the detection of metal-EDTA complexes. The vancomycin concentration, phosphate concentration and pH of the buffer strongly influenced mobility, resolution and selectivity of the studied analytes. Under the optimal condition, the relative standard deviations （n=5） of the migration time and the peak area were less than 3.14% and 7.35%, respectively. Application of the Z-shape cell capillary electrophoresis method with UV detection and vancomycin loading led to the reliable determination of these metal ions in tap water and the recoveries were 97%-101%. The detection limits based on a signal to noise ratio of 3 ： 1 were found in the range of 2-10 μg·L^-1.

关 键 词：Z-shape cell capillary electrophoresis VANCOMYCIN metal ion ethylenediamine tetraacetic acid 

分 类 号：O657.8[理学—分析化学]