不同疾病来源幽门螺杆菌菌株对人胃癌细胞系AGS基质金属蛋白酶表达影响的比较研究  

Effects of Helicobacter pylori Strains from Different Disease Origins on Expression of Matrix Metalloproteinases in Human Gastric Cancer Cell Line AGS:a Comparative Study

作　　者：王继恒[1] 刘文忠[2] 韩跃华[3] 童菊芳[2] 朱红音[2] 李恩灵[2] 

机构地区：[1]北京军区总医院消化内科,100700 [2]上海交通大学医学院附属仁济医院消化内科上海市消化疾病研究所 [3]浙江大学医学院附属第二医院消化科

出　　处：《胃肠病学》2006年第11期657-662,共6页Chinese Journal of Gastroenterology

摘　　要：背景：幽门螺杆菌(H．pylori)是胃癌的Ⅰ类致癌原,但H．pylori感染与胃癌发生的分子机制仍不明了。目的：在体外观察不同疾病来源的H．pylori菌株对人胃癌细胞系AGS基质金属蛋白酶(MMPs)表达的影响是否存在差异。方法：将AGS细胞分别与5株分离自胃癌和5株分离自轻度非萎缩性胃炎患者的H．pylori共培养,以逆转录聚合酶链反应(RT-PCR)和蛋白质印迹法(Western blot)检测MMP-2、MMP-7和MMP-9 mRNA和蛋白的表达,以肠道致病性大肠杆菌作为细菌对照。结果：胃炎H．pylori菌株和大肠杆菌基本不影响AGS细胞MMP-2、MMP-7和MMP-9 mRNA和蛋白的表达,而所有胃癌菌株均能上调MMP-2、MMP-7和MMP-9 mRNA和蛋白的表达。结论：分离自胃癌和胃炎患者的H．pylori菌株对AGS细胞MMP-2、MMP-7和MMP-9表达的影响有所不同,说明不同疾病来源的H．pylori菌株在促细胞恶变能力上存在差异。Background： Helicobacter pylori （H. pylori） is class Ⅰ carcinogen for gastric cancer. However, the molecular mechanisms linking H. pylori infection to development of gastric cancer remain unclear. Aims： To observe the effects of H. pylori strains from different disease origins on the expression of matrix metalloproteinases （MMPs） in human gastric cancer cell line AGS in vitro. Methods： AGS cells were co-cultured with ten strains of H. pylori, five from patients with gastric cancer and five from those with mild non-atrophic gastritis, respectively. Expressions of MMP-2, MMP-7 and MMP-9 at both mRNA and protein levels were determined by reverse transcriptase polymerase chain reaction （RT-PCR） and Western blot, and the enteropathogenic Escherichia coil （E. coli） was used as a bacterial control. Results： E. coli and H. pylori strains from gastritis patients had no effects on the expressions of MMP-2, MMP-7 and MMP-9 in AGS cells both at mRNA and protein levels on the whole. In contrast, all the H. pylori strains from gastric cancer patients increased remarkably the expressions of MMP-2, MMP-7 and MMP-9 at both mRNA and protein levels. Conclusions： The H. pylori strains isolated from gastric cancer and gastritis patients show different effects on the expressions of MMP-2, MMP-7 and MMP-9 in AGS cells. These results suggest that there exist discrepancies in the ability of promoting cellular malignant transformation between H. pylori strains from different disease origins.

关 键 词：螺杆菌 幽门 细胞系/AGS 基质金属蛋白酶类 

分 类 号：R735.2[医药卫生—肿瘤]