高压氧对实验性高眼压鼠视网膜细胞色素氧化酶和一氧化氮合酶的影响  

Influences of hyperbaric oxygenation therapy on cytochrome oxidase and nitric oxide synthase in retina of rats with experimentally elevated intraocular pressure

作　　者：易军晖[1] 彭争荣[2] 刘求理[3] 吴钟琪[2] 

机构地区：[1]中南大学湘雅三医院眼科,湖南省长沙市410013 [2]中南大学湘雅医院高压氧科,湖南省长沙市410018 [3]中南大学湘雅医学院解剖教研室,湖南省长沙市410013

出　　处：《中国临床康复》2006年第46期114-116,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察高压氧对急性实验性高眼压大鼠视网膜损伤后的细胞色素氧化酶和一氧化氮合酶恢复的促进作用。方法押实验于2005-03/08在中南大学湘雅医院高压氧科及中南大学湘雅医学院解剖实验室进行。取健康Wistar大鼠25只,单纯随机分成3组:①模型组9只鼠,双眼用生理盐水前房灌注加压至视网膜电图b波消失90min制作高眼压模型,造模后不做任何治疗。②高压氧组9只鼠,双眼制作高眼压模型后行0.2MPa高压氧治疗,吸高浓度氧80min,平均氧体积分数为0.8252±0.0258,1次/d,共治疗7次。③空白对照组7只鼠,仅行角膜穿刺。实验结束后取双侧眼球视网膜进行测定,采用HPIAS-1000型计算机图像分析系统进行细胞色素氧化酶和一氧化氮合酶反应的定量分析,指标包括阳性细胞数,阳性产物的吸光度、体密度和平均截面积,并进行相关性分析。结果押25只大鼠全部进入结果分析。①高压氧组的NADPH/一氧化氮合酶阳性细胞数,阳性产物的吸光度、体密度、平均截面积均高于模型组眼(13.67±2.40),(5.00±2.92)个/视野;0.137±0.015,0.088±0.028;(0.52±0.12)%,(0.22±0.12)%;(74.00±4.61),(63.44±7.02)μm2;P均<0.01演。②高压氧组的细胞色素氧化酶阳性细胞数,阳性产物的吸光度、体密度、平均截面积均高于模型组眼(13.44±3.05),(4.67±2.12)个/视野;0.123±0.026,0.089±0.035;(0.859±0.30)%,(0.182±0.068)%;(99.89±6.05),(68.78±10.16)μm2;P均<0.05演。③治疗后一氧化氮合酶活性和细胞色素氧化酶活性的4项指标呈正相关(P<0.01)。结论押高压氧治疗可提高视网膜缺血组织的细胞色素氧化酶和一氧化氮合酶活性,提示高压氧能保护视神经细胞抵抗缺血性损伤。AIM： To observe the promotion of hyperbaric oxygenation therapy on cytochrome oxidase （CO） and nitric oxide synthase （NOS） in rats with experimentally acute elevated intraoeular pressure （IOP）. METHODS： The experiment was done in the Department of Hyperbaric Oxygenation, Xiangya Hospital, Central South University and the Laboratory of Anatomy, Xiangya Medical College, Central South University from March to August 2005. Twenty-five healthy Wistar rats were divided into 3 groups randomly： ① Model group included nine rats. Acute elevated IOP models were built after b wave disappearing for 90 minutes by perfusion of saline. No other therapy was done after establishing models. ② Hyperbaric oxygenation group included 9 rats. After establishing elevated IOP models, the rats received 0.2 MPa hyperbaric oxygenation therapy and sucked in oxygen of high concentration for 80 minutes, average volume fraction of 0.825 2±0.025 8, once a day for 7 times. ③ Seven rats in the blank control group only received keratonyxis. Retina of both sides was measured after experiment. Quantitative analysis of NOS and CO was performed with HP1AS-1000 computer image analysis, including positive cell population, absorbanee （A）, bulk density and mean section area of positive product. Correlation analysis was also conducted. RESULTS： Totally 25 rats were involved in the result analysis. ① NADPH/NOS positive cell population, A, bulk density and mean section area of positive product in the hyperbaric oxygenation group were higher than those in the model group [（13.67±2.40）, （5.00±2.92） pieces per field; 0.137±0.015, 0.088±0.028; （0.52±0.12）%, （0.22±0.12）%; （74.00±4.61）, （63.44±7.02） μm^2; P 〈 0.01]. ② CO positive cell population, A, bulk density and mean section area of positive product in the hyperbarie oxygenation group were higher than those in the model group [（13.44±3.05）, （4.67±2.12） pieces per field; 0.123±0.026, 0.089±0.035; （0.859±0.30）%, （0.182±0.0

关 键 词：高压氧 细胞色素C氧化酶 一氧化氮合酶 高眼压 疾病模型 动物 

分 类 号：R77[医药卫生—眼科]