家兔血管神经末梢感受器通路与皮肤末梢感受器通路的关系  

Relationship between the pathway of vascular nerve terminal receptor and the pathway of cutaneous terminal receptor in rabbits

作　　者：章云海[1] 雷玲[1] 耿祝生[1] 徐志强[1] 李静[1] 扬聪颖[2] 李玉[3] 曾因明[4] 周震球[1] 李爱民[5] 刘克芳[1] 陆爱蓉[1] 

机构地区：[1]连云港市第一人民医院麻醉科,江苏省连云港市222002 [2]连云港市第一人民医院病理科,江苏省连云港市222002 [3]连云港市第一人民医院肿瘤生物实验室,江苏省连云港市222002 [4]连云港市第一人民医院神经外科,江苏省连云港市222002 [5]江苏省麻醉学重点实验室,江苏省徐州市2210021

出　　处：《中国临床康复》2006年第46期141-144,共4页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助(30271260);江苏高校省级重点实验室第三批对外开放课题(K2093);江苏省科技厅指导计划课题(BS2002305);连云港市科技局课题(SH0408);连云港市科技局课题(SH0507)~~

摘　　要：目的:为进一步明确血管神经末梢感受器通路与皮肤末梢感受器通路的关系,从家兔血管入手,用形态学方法来观察分析血管的感受器通路网络。方法:实验于2004-10/11在连云港市第一人民医院肿瘤生物实验室进行。①选取雄性家兔4只,随机数字表法分为神经逆行追踪18～24h组、神经逆行追踪30～36h组,2只/组。②两组兔以乌拉坦1.5g/kg麻醉后,经耳缘静脉注射1g/L荧光素核黄(Sigma,批号74681-68-8)500μL逆行神经追踪,神经逆行追踪18～24h组规定兔成活18～24h,神经逆行追踪30～36h组规定兔成活30～36h。③分别取C2～L5脊神经节、颈、胸交感神经节、肠系膜下神经节、大脑、小脑、丘脑、脑干、脊髓、胃、小肠、心肌、肺、肾、骶脊肌、肠系膜下动脉、牵涉区部位的皮、骶部的皮及耳,冰冻连续切片,分别做成两套,一套蒸馏水贴片,直接在OlympusZX-004荧光显微镜下寻找各组织荧光标记的神经细胞、荧光标记密集的神经末梢部位;另一套行苏木精-伊红染色后再置于荧光显微镜下,观察有无新的发现。结果:4只兔均进入结果分析。①神经逆行追踪18～24h组:在颈、胸交感神经节及肠系膜下神经节发现标记细胞;在胃黏膜下标记到副交感神经元;在小肠黏膜及黏膜下标记到大量的节细胞;在C2～L5脊神经节未发现标记细胞,但在神经节外膜上可见密集的荧光;在脊髓前、后角未见到标记细胞及明显的荧光密集区,但有较弥散的荧光;在下丘脑、大脑及小脑未发现标记细胞及明显的荧光密集区,骶脊肌膜、气管内外膜、心肌的内外膜、肾小球、皮内毛细血管袢发现较明显的荧光密集区;肠系膜动脉壁发现密集荧光。苏木精-伊红染色后,肠系膜下神经节有标记细胞,细胞核为金黄色,细胞质为蓝黑色,在胃黏膜下标记到副交感神经元。②神经逆行追踪30～36h组:在C2～L5脊神经节未发现核黄标记�AIM： To further identify the relationship between pathway of vascular nerve terminal receptor and pathway of cutaneous terminal receptor, and analyze the pathway net of vascular receptor by means of morphological method in rabbits. METHODS： The experiment was conducted in the Biochemical Laboratory of Tumor, First People＇s Hospital of Lianyugang from October to November 2004. ① Four female rabbits were selected and divided randomly into retrograde neural tracing 18-24 hours group and 30-36 hours group with two rabbits in each group. ② Rabbits of the two groups were injected with 1 g/L fluorescent riboflavin （Sigma, 74681-68-8） in the vein of ear edges under urethane anesthesia and then received 500 μL retrograde neural tracing. The rabbits in the retrograde neural tracing 18-24 hours group survived 18-24 hours, and rabbits in the retrograde neural tracing 30-36 hours group survived 30-36 hours. ③ The frozen serial sections were made in spinal ganglia from C2 to Ls, cervical and thoracic sympathetic ganglia, inferior mesenteric ganglion, cerebrum, cerebellum, thalamus, brain stem, spinal cord, stomach, small intestine, myocardium, lung, kidney, sacral spinal muscle in sacrum, inferior mesenteric artery, dermis in the referral area, dermis and ear in sacrum respectively. The sections were made into 2 series： one was stuck in the distilled water, then observed under an Olympus ZX-004 fluorescence microscope directly to find fluorescence marked neuron and fluorescence dense area of nerve terminal. The other was stained with hematoxylin and eosin, then observed under the fluorescence microscope to determine whether there was new discovery. RESULTS： Four rabbits were involved in the result analysis. ① Retrograde neural tracing 18-24 hours group： Labeled cells were found in cervical, thoracic sympathetic ganglia and inferior mesenteric ganglion. Parasympathetic neurons were labeled below gastric mucous membrane. A lot of ganglion cells were labeled on and below of small intestinal mucous mem

关 键 词：血管 神经节 交感 神经节 副交感 经络 

分 类 号：R741.02[医药卫生—神经病学与精神病学]