血清、脑脊液肿瘤坏死因子-α水平及其-308G/A基因多态性与多发性硬化的相关性  

Association among serous and cerebrospinal fluid TNF-α level,gene polymorphisms of TNF-α and multiple sclerosis in Han nationality of southern China

作　　者：董亚贤[1] 许志荣 林佩玉[1] 

机构地区：[1]广州医学院第一附属医院神经内科,510120 [2]广东省交通医院内科

出　　处：《中华医学遗传学杂志》2006年第6期677-679,共3页Chinese Journal of Medical Genetics

摘　　要：目的研究中国广东汉族人群中血清、脑脊液(cerebrospinalfluid,CSF)肿瘤坏死因子-α(tumornecrosisfactor-α,TNF-α)水平及其-308G/A基因多态性与多发性硬化之间的相关性。方法采用双抗体夹心ABC-ELISA法测定68例无亲缘关系的急性发作期多发性硬化(multiplesclerosis,MS)患者和55例非免疫系统疾病患者的血清、CSF的TNF-α水平,同时应用聚合酶链反应-限制性片段长度多态性技术检测上述68例MS患者和106名无血缘关系的广东籍健康汉族人的TNF-α-308G/A基因型。结果发作期MS患者血清中TNF-α水平与非免疫系统疾病患者组间差异有统计学意义(P<0·05),分别是(276±71)pg/mL和(234±76)pg/mL;发作期MS患者CSF中TNF-α水平与非免疫系统疾病患者组差异无统计学意义(P>0·05),分别是(265±78)pg/mL和(245±83)pg/mL;TNF-α-308G/A各等位基因型频率在MS组和正常人组比较差异无统计学意义(P>0·05),MS组TNF-α基因AA基因型和A等位基因频率分别为4.4%和14·0%,正常人对照组分别为0和8.5%。结论(1)发作期MS与血清中TNF-α水平相关,与CSF中TNF-α水平不相关。(2)从目前调查的例数中,中国广东汉族人群TNF-α基因-308G/A多态性与广东人群中MS不相关。Objective To investigate the association among serous and cerebrespinal fluid （CSF） TNF-α level, gene polymorphisms of TNF-α and multiple sclerosis （MS） in Hart nationality of southern China. Methods MS diagnosis was base on Poser （1983） criteria. Fifty-five patients with nonimmulogical diseases and 68 patients with MS from seuthern China were enrolled in the study, and their TNF-α level of serum and CSF were measured by double antibody sandwich ABC-ELISA. TNF-α - 308G/A in 106 normal healthy subjects and 68 MS patients was genotyped with polymerase chain reaction-restriction fragment length polymorphism. Results There was significant difference in the serous TNF-α level between nonimmune patients and active MS patients （234±76 pg/mL vs 276±71 pg/mL, P 〈 0.05）, but not in the CSF （245±83 pg/mL vs 265±78 pg/mL, P 〉 0.05）. The gene frequency distribution of TNF-α - 308G/A was corresponding with Hardy-Weinberg equilibrium. The positive rate of genotype AA and the gene frequency of allele A of TNF-α were 4.4% and 14.0% in MS group, and 0 and 8.50% in healthy subjects, there was no statistical significance （P 〉 0.05）. Conclusion The TNF-α level in serum is associated with active MS, but not in the CSF. The gene polymorphisms of TNF-α - 308G/A is not associated with MS in Han nationality of southern China.

关 键 词：多发性硬化 肿瘤坏死因子-Α 遗传多态性 

分 类 号：R744.51[医药卫生—神经病学与精神病学]