实验性兔动脉瘤氧化应激评价  

Evaluation of Oxidative Stress in Experimental Rabbit Aneurysms

作　　者：李立[1] 杨新健[1] 蒋凡[1] 刘爱华[1] 吴中学[1] Gregory J Dusting 

机构地区：[1]北京市神经外科研究所神经介入科,北京100050 [2]Howard Florey Institute,the University of Melbourne,Victoria 3010,Australia

出　　处：《中国医学科学院学报》2006年第5期707-709,共3页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(30200289);北京市自然科学基金(7022008);北京市科技新星培养计划(H020820950130) ~~

摘　　要：目的评价氧化应激在动脉瘤发生发展中的作用。方法复制兔顶端动脉瘤模型5个和侧方动脉瘤模型8个,以6段正常血管为对照。用化学方法检测标本中丙二醛(MDA)、超氧化物歧化酶(SOD)和抗活性氧单位的水平。结果顶端动脉瘤和侧方动脉瘤标本中MDA含量分别为(33.85±8.66)和(27.87±5.78)nmol/mgprot,均显著高于对照组的(10.91±2.72)nmol/mgprot(P均<0.01);两种动脉瘤的SOD含量分别为(28.30±3.58)和(33.00±8.09)U/mgprot,均显著低于对照组(127.27±38.72)U/mgprot(P均<0.01);两种动脉瘤的抗活性氧单位含量分别为(47.86±5.00)和(62.64±13.87)U/mgprot,也均显著低于对照组(116.94±9.22)U/mgprot(P均<0.01)。顶端动脉瘤和侧方动脉瘤之间除抗活性氧单位具有显著差异(P=0.014)外,MDA和SOD均无统计学差异。MDA与SOD和抗活性氧单位呈显著负相关关系,相关系数分别为-0.830和-0.852(P<0.01)。结论氧化应激对血管造成的损伤可能在动脉瘤的发展过程中起重要作用。不同类型动脉瘤的氧化应激差异性不明显。Objective To evaluate the roles of oxidative stress in the generation and development of aneurysms. Methods Five terminal aneurysms and 8 lateral aneurysms were rebuilt on rabbits, and 6 normal artery vessles were prepared as control. The levels of malondialdehyde （MDA） , superoxide dismutase （SOD） , and anti-reactive oxygen species unit （ anti-ROS unit） were measured with chemical methods. Results The levels of MDA in terminal and lateral aneurysms were （ 33.85 ± 8.66 ） and （27.87 ± 5.78 ） nmol/mg prot, respectively, which were significantly higher than （10. 91 ± 2. 72） nmol/mg prot in control group （P 〈 0. 01 ）. The levels of SOD in terminal and lateral aneurysms were （28.30 ± 3.58 ） and （ 33.00 ± 8.09 ） U/mg prot, respectively, which were significantly lower than （ 127.27 ± 38. 72） U/mg prot in control group （ P 〈 0. 01 ）. The levels of anti-ROS unit in terminal and lateral aneurysms were （47.86 ± 5.00） and （62. 64 ± 13.87 ） U/ mg prot, respectively, which were significantly lower than （ 116. 94 ± 9. 22） U/mg prot in control group （ P 〈 0. 01 ）. No significant differences were shown between terminal aneurysm and lateral aneurysm in MDA and SOD except anti-ROS unit （P =0. 014）. MDA had negative correlations with both SOD and anti-ROS unit, and the correlation coefficients were -0. 830 and -0. 852, respectively. Conclusion Oxidative stress may play an important role in the development of aneurysms. Oxidative stress seems similar among various aneurysms.

关 键 词：动脉瘤 氧化应激 自由基 

分 类 号：R743[医药卫生—神经病学与精神病学] R362[医药卫生—临床医学]