二硫化碳对血管内皮细胞的氧化损伤作用  

The Effect of Carbon Disulfide on Oxidative Injury in Vein Endothelial Cells

作　　者：陈绍兰[1] 简乐[2] 王菁[2] 陈苘[2] 朗惠卿[2] 

机构地区：[1]杭州市萧山区疾病预防控制中心,浙江杭州311201 [2]浙江大学医学院劳动卫生与环境卫生研究所

出　　处：《浙江预防医学》2006年第12期8-9,12,共3页Zhejiang Journal of Preventive Medicine

摘　　要：目的为了探讨二硫化碳(CS2)致动脉粥样硬化(AS)的毒作用机理。方法体外培养人脐静脉内皮细胞(ECV304)用浓度0(溶剂对照)、10、100、1000、10000μmol/L的CS2染毒,共培养24h。应用倒置显微镜观察内皮细胞(EC)损伤;鲁米诺依赖的化学发光法(L-CL)测定培养液过氧亚硝基阴离子(ONOO-)水平以观察细胞氧化损伤程度。结果镜下观察染毒24h后内皮细胞有不同程度的损伤,其中以10000μmol/L CS2染毒组最为严重,并有明显细胞脱壁现象;24h染毒培养液化学发光强度有随着CS2浓度增高而增强的趋势(F=34.60,P<0.05),其中10000μmol/L CS2染毒组明显高于其它各组(P<0.05);CS2浓度与内皮细胞培养液ONOO-水平呈正相关(r=0.998,P<0.05)。结论CS2可促使内皮细胞产生大量自由基,包括ONOO-,以至氧化抗氧化失衡,从而导致血管内皮细胞的氧化损伤。Objective To study the toxic mechanism of atherosclerosis induced by Carbon disulfide （CS2）, Methods Human umbilical vein endothelial cells （ECV304） were cultured for 24 hours with different level of CS2 exposure （ 10, 100, 1000, 10000μmol/L and 0 μmol/L as a control group）. Microscope was used to observe the morphological injury of endothelial cells and the method of Luminol - dependent chemiluminescence was adopted to measure the level of peroxynitrite anion of cell culture medium to observe the oxidative injury. Results Morphological injury in endothelial cells to a different extent was observed by microscope. The level of peroxynitrite in cell culture medimn with CS2 was significantly higher than that without CS2 （P 〈 0.05）. The concentration of CS2 positively correlated with that of peroxynitrite anion （P 〈 0.05）. Conclusions CS2 can induce the oxidative injury in endothelial cells by producing free radicals including peroxynitrite anion.

关 键 词：二硫化碳 内皮细胞 过氧亚硝基阴离子 氧化损伤 

分 类 号：R-332[医药卫生]