测定维生素B_1含量的HPLC法  

Content detection of vitamin B_1 by HPLC

作　　者：袁冬梅[1] 刘玲玲[2] 

机构地区：[1]天水师范学院化学系,甘肃天水741001 [2]兰州师范高等专科学校化学系,甘肃兰州730070

出　　处：《中国酿造》2006年第12期57-58,共2页China Brewing

摘　　要：以C18为分离柱,以100mmol/L磷酸钠缓冲溶液(pH2.1)/0.8mol/L辛基磺酸和乙腈=9:1(v/v)混合液为流动相,检测波长为270nm的液相色谱检测VB1含量,在0.005μg￣2.5μg范围内进样与主峰面积线性关系良好(r=0.9997),最小检测量0.0050μg,检出限0.0002μg。The content of vitamin B1 （VB1） was detected by high performance liquid chromatography （HPLC） at 270 nm, in which C18 Chromatography column was used. The mobile phase was a mixture containing acetonitrile and the solution of 100 mmol/L Na3PO4 （pH 2.1） and 0.8 mol/L octylsulfoacid, with the mix ratio of 1：9 （v/v）. The content of VBI and the area of main peak presented good linear relationship （r=0.9997） when the content of VBI ranged between 0.005 μg and 2.5 μg. The minimum injection dose was 0.0050 μg, and the detection limit was 0.0002 μg.

关 键 词：VB1 高效液相色谱法 含量 

分 类 号：O657.7[理学—分析化学]