胶滴肿瘤药敏检测技术(CD-DST)的建立及初步临床应用研究  

The establishment of collagen gel droplet culture drug-sensitivity test(CD-DST)techniques and its clinical application

作　　者：梁智[1] 田海梅[1] 李艳芬[1] 曹冬艳[1] 刘毅[1] 李茉[1] 王小兵[1] 程冬婉[1] 张伟[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院肿瘤研究所生物检测中心,北京100021

出　　处：《癌症进展》2006年第6期545-549,共5页Oncology Progress

摘　　要：目的建立胶滴肿瘤体外药敏检测技术,探讨其在肿瘤个体化治疗中应用的可行性。方法用人类肿瘤细胞系建立胶滴肿瘤体外药敏检测技术,并用该技术对50例不同肿瘤类型的原代肿瘤标本进行体外药敏检测实验研究,每种肿瘤标本分别进行了4～5种抗肿瘤药物的敏感性测定。结果在体外成功建立了胶滴肿瘤药敏检测技术,标本整体评价率为82.0%(41/50),检测结果与临床经验有效率间有较好的符合性。结论CD-DST体外肿瘤药敏检测技术是一种较好的药敏检测方法,标本可评价率为82.0%(41/50),所需标本量小,可反映病人对不同抗肿瘤药物敏感性的差异,检测结果与临床经验有效率有较好的符合性,对抗肿瘤药物的体外筛选和对个体化治疗具有实际应用价值。Objective To establish Collagen gel droplet culture and explore its clinical feasibility of application. Methods drug - sensitivity test （ CD - DST） technique The human tumor cell lines were used to find out the best cuhure conditions of collagen gel droplet culture drug - sensitivity test （ CD - DST）. The chemosensitivity of 4 or 5 anticancer drugs was assessed by the CD - DST in the ovarian and uterine cancer （n =18）, gastrointestinal cancer （n=15）, lymphoma （n =8）, lung cancer （n=5）, liver cancer （n= 4）. Results We established the collagen gel droplet culture drug - sensitivity test （ CD - DST） by using the human tumor cell lines. The CD - DST can evaluate the efficacy of anticancer drugs in diverse kinds of tumor specimen. There was a statistically correlation between clinically reported response rates and the re- sponse rates obtained by CD- DST. The overall tumor evaluation rate was 82. 0% （41/50）. Conclusion The CD - DST is a good chemosensitivity assay methods with the advantage of high overall predicting value and a comparatively small number of ceils, so that it can solve the issue of minor specimen source. Our re- sults in showed the in vitro sensitivity for each drug is a significant correlation with clinically reported re- sponse rates. It appears to be useful in exploring the possible combination plots and principles, evaluating the efficacy of existing chemotherapy, and optimize chemotherapy on an individual basis.

关 键 词：肿瘤 药敏检测 胶滴肿瘤药敏检测技术 

分 类 号：R73-361[医药卫生—肿瘤]