肾组织中核心蛋白聚糖的表达和定位及其与肾间质损害的关系  

Expression and orientation of decorin in renal tissue and correlalionship between decorin and tubulointerstitial damage

作　　者：成学琴[1] 鲍华英[1] 陈颖[1] 郭梅[2] 

机构地区：[1]南京医科大学附属南京儿童医院肾脏内科,江苏南京210008 [2]南京医科大学儿科医学研究所,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2006年第12期1248-1251,F0004,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京市科技局人才培养项目(200306062)资助

摘　　要：目的:观察核心蛋白聚糖在小儿肾脏病肾活检组织中的定位及表达,并探讨其与肾小管间质损害程度及转化生长因子-β1(TGF-β1)之间的关系。方法:收集36例肾脏疾病患儿肾活检病理及临床资料,对其肾小管间质损害程度进行分组,应用免疫组织化学方法检测核心蛋白聚糖和TGF-β1在肾间质内的定位及表达。结果:核心蛋白聚糖主要表达于肾小管上皮细胞,近曲小管表达比远曲小管更为明显,随着肾间质损害程度的加重,核心蛋白聚糖的表达量逐渐增加。TGF-β1的表达亦主要见于肾小管上皮细胞,在近曲小管和远曲小管之间无明显的差异,TGF-β1的表达量也随着肾小管间质损害程度的加重而增加。肾间质中,核心蛋白聚糖和TGF-β1两者表达量呈正相关。结论:核心蛋白聚糖可能作为反映肾间质损害程度的一个标志性蛋白,同时对TGF-β1的表达起一定的抑制作用。Objective： To investigate the expression and orientation of decorin in renal biopsy and to investigate the correlation between decorin and TGF-β1 and renal tubulointerstitial fibrosis. Methods：The pathological and clinical data of 36 patients with glomerulonephritis and tubulointerstitial diseases were collocted, and divided into different groups according to serious degrees. The expression and orientation of decorin and TGF-β1 in the renal tissue were detected by immunochemistry. Results：Decorin was mainly expressed in renal tubular epithelial cells, especially in epithelium of proximal tubule. With the development of tubulointerstitial lesions, the expression of decorin was increased. TGF-β1 was mainly expressed in renal tubular epithelial cells, and there was no differece between proximal tubule and distant tubule. The expression of TGF-β1 was also increased with the development of tubulointerstitial lesions. A positive correlation was found between decorin and TGF-β1 in renal tubule. Conelusion：Decorin can take as an indicator to response the tuhulointerstitial damage, at the same time, it can reduce the expression of TGF-β1 in some degree.

关 键 词：核心蛋白聚糖 肾间质损害 转化生长因子-Β1 

分 类 号：R365.69[医药卫生—病理学] Q786[医药卫生—基础医学]