4－甲基组胺对HL＿（60）细胞增殖、分化及c－myc基因表达的影响  

THE EFFECTS OF 4-MH ON THE PROLIFERATION,DIFFEKENTIATION AND C-myc ONCOGENE EXPRESSION OF HTE HL60 PROMYELOCYTIC LEUKEMIA CELLS

作　　者：刘建英 贺石林 蒋德昭 

出　　处：《湖南医科大学学报》1996年第5期375-377,共3页Bulletin of Hunan Medical University

基　　金：国家自然科学基金

摘　　要：用体外液体培养法，观察组胺Ｈ＿２受体激动剂４－ＭＮ对人类早幼粒白血病细胞系ＨＬ＿（６０）细胞的增殖和分化的影响。结果表明，１０￣（－３）～１０￣（－５）ｍｏｌ·Ｌ￣（－１）的４－ＭＨ能抑制ＨＬ＿（６０）细胞增殖，促进其分化。用胞浆ＲＮＡ斑点杂交技术检测到１０￣（－４）ｍｏｌ·Ｌ￣（－４）４－ＭＨ能降低ＨＬ＿（６０）细胞ｃ－ｍｙｃ基因的ｍＲＮＡ表达。he effects of 4-MH(4-methylhistamine,an agonist of histamine H2-receptor)on the prolif-eration and differentiation of the HL60 promyelocytic leukemia cell line were investigated in theliquid cuIture in vitro.It showed that 4-MH at concentrations ranging from 10-5mol·L-1 to 10-3mol·L-1 significantly inhibited the proliferation and induced the differentiation of HL60 cells(P<0.05),The dot blot technique of total cellular RNA with α-32 P nicktranslated c-myc gene asprobe was used to study the changes in transcription level of c-myc oncogene as HL60 leukemiccells were treated bv 4-MH.The results showed that 4-MH 10-4 mol·L-1 could decrease level ofc-myc mRNA and inhibit the transcription of c-myc mRNA in HL60 cells.

关 键 词：甲基组胺 组胺 激动剂 HL60细胞 C-MYC基因 

分 类 号：R363[医药卫生—病理学] R733.702[医药卫生—基础医学]