Rb对HepG_2肝癌细胞系增殖的影响  

Effect of Rb on Proliferation of HepG_2 Cell Line

作　　者：刘清源[1] 施学忠[1] 李来生[2] 孙斌[1] 

机构地区：[1]郑州大学医学院,郑州市450052 [2]中国人民武装警察部队河南省总队医院,郑州市450052

出　　处：《医药论坛杂志》2006年第22期10-11,共2页Journal of Medical Forum

摘　　要：目的研究Rb基因及其产物对HepG2肝癌细胞系增殖的影响。方法转染pRb质粒进入HepG2肝癌细胞系,用MTT法检测转染前后细胞增殖的变化,并将转染pRb质粒的HepG2肝癌细胞接种在裸鼠皮下建立肝癌裸鼠种植瘤模型,观察转染pRb质粒前后裸鼠体内种植瘤生长的变化。结果转染pRb质粒的HepG2肝癌细胞与未转染pRb质粒者相比细胞增殖受到抑制。成功建立HepG2肝癌细胞裸鼠体内种植瘤模型,转染pRb质粒的HepG2肝癌细胞的裸鼠体内种植瘤生长受抑制。转染组种植瘤体积第4周(429.7±114.987)mm3,第5周(657.90±187.27)mm3,第6周(892.56±258.73)mm3。结论Rb在体内和体外均能抑制肝癌细胞的增殖。Objective To study the effect of Rb on proliferation of HepG2 cell line. Methods Transfect pRb plasmid into HepG2 cell line, then measure the growth change after transfection by MTT assay. To establish the transplanted liver cancer nude mice model, then measure the growth change of the transplanted tumor after being transfected with pRb plasmid. Results The proliferation of HepG2 cell line transfected with pRb plasmid was inhibited. Successfully established the liver cancer transplanted nude mice model, the growth of cancer was inhibited in the nude mice transplanted with HepG2 cell line which transfected with pRb plasmid. Conclusion Rb can inhibit the growth of HepG2 cell line in vivo and in vitro.

关 键 词：RB 肝癌 细胞系 增殖 

分 类 号：R735.7[医药卫生—肿瘤]