卵巢癌肿瘤浸润淋巴细胞的临床研究  

ClinicalStudyonImmunotherapyofOvarianCancerwithTumorInfiltratingLymphocytes

作　　者：华祖德[1] 陆静[1] 李慧芳[1] 李彪如 朱佑明 童庆善 

机构地区：[1]上海第二医科大学附属瑞金医院妇产科

出　　处：《中华妇产科杂志》1996年第9期555-557,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的：探讨卵巢癌肿瘤浸润淋巴细胞的临床应用。方法：对１５例卵巢癌患者给予化疗及肿瘤浸润淋巴细胞（ＴＩＬ）治疗（治疗组），另对１０例卵巢癌患者仅给予化疗（对照组）。观察ＴＩＬ的分布及血清ＣＡ１２５的变化。结果：卵巢癌组织表面浸润结节、实质部位及卵巢区域３个区域中，表面浸润结节的ＴＩＬ数量最多，为７．２４×１０５个／ｇ。实质部位为２．４９×１０５个／ｇ，最少的是卵巢区域，为２．０２×１０５个／ｇ；表面浸润结节、卵巢区域及实质部位的扩增倍数分别为３０００、２５００及２０００。表面浸润结节的ＴＩＬ杀伤活力也最高。经ＴＩＬ治疗后，卵巢癌患者的ＣＡ１２５显著下降，肿块缩小或消失，腹水完全消失。结论：卵巢癌表面浸润结节的ＴＩＬ数量及杀伤活力明显高于实质部位及卵巢区域，是获得ＴＩＬ的最佳取材部位。Objective:Toinvestigateclinicalusageofovariantumorinfiltratinglymphocytes.Methods:Tumorinfiltratinglymphocytes(TIL)wereisolatedbycolddigestionmethod,specimensbeingtakenfrom:(1)infiltratingnodeontumorsurface.(2)thetumorproper.(3)theovariantis-sue.Arandomized,doule-blindstudyontwogroupsonovariancancer,totaly25patientswascon-ducted.ThefirstgroupwasgivenTILandchemotherapyandthesecondgroupwasgivenchemother-apyonly.Result:Theyieldsofinfiltratingnodeontumorsurfacewerethehighest(7.24×105),inthetumorproper(2.49×105),andintheovariantisuethelowest(2.07×105).GrowthofTILinnodesontumorsurfaceincreased3000fold,inovariantissuesandintumorproper2500and2000timesrespectively.Themosteficientkilingactivitywasalsopresentedintheinfiltratingnodesontumorsurface.CA125decreasedsignificantly,tumorregressedintwopatientsonfolow-upbyultra-sound,andascitesdisappearedcompletelyafteronecourseofchemotherapyandTILsinfusion.Con-clusion:TheyieldsofTILininfiltratingnodesontumorsurfaceandtheireficiencyinkilingactivitywerehigherthanthoseintumorproperandovariantisues,andtheinfiltratingnodesontumorsur-facewereconsideredthebestsitesforobtainingspecimens.

关 键 词：卵巢肿瘤 淋巴细胞 肿瘤浸润 

分 类 号：R737.31[医药卫生—肿瘤]