大豆异黄酮糖苷酶产生菌培养条件优化及转化  

Cultural conditions and enzymolysis of soybean isoflavone-glycosidase

作　　者：王振丽[1] 李莉[2] 李剑梅[2] 高群[1] 于艳敏[1] 

机构地区：[1]沈阳农业大学土地与环境学院,辽宁沈阳110161 [2]辽宁省微生物科学研究院,辽宁朝阳122000

出　　处：《中国微生态学杂志》2006年第6期444-445,共2页Chinese Journal of Microecology

摘　　要：将经过筛选的产大豆异黄酮糖苷水解酶的菌株米曲霉3042经过单因子及正交试验,确立了产酶的最适培养基配方为:玉米芯+麸皮4%,(NH4)2SO40.1%,水扬苷0.01%,KH2PO40.1%,Vc 0.1%,MgSO40.1%。产酶的最佳培养条件为:发酵培养基起始pH 6.0,发酵温度27℃,摇床转速160 r/min,发酵时间84 h时酶活力最高。粗提取的大豆异黄酮经发酵液转化后,其结合态含量降低,游离态含量增加。An Aspergillus oryzae producing β-glucosidase was selected. Its medium ingredients and termentation condions were optimized by single factor and orthogonal experiments. The results were as follows： corncob and wheat bran 4%, （NH4）2SO4 0.1% ,salicin 0.01% ,KH2PO4 0.1% ,Vc 0.1% ,MgSO4 0.1%. The best cultivation condition of producing enzyme was pH 6.0,temperature 27 ℃, 160 r/mino The best fermentation time was 84 h. The soybean isoflavone was hydrolyzed with soybean isoflavone-glycosidase,the content of combination was reduce and the content of dissociate was increased.

关 键 词：大豆异黄酮糖苷水解酶 米曲霉 产酶条件 转化 

分 类 号：Q556.2[生物学—生物化学]