姜黄素对两株不同转移力小鼠腹水型肝癌细胞生物学特性的影响  

Effects of curcumin on biological behavior of two murine ascites hepatocarcinoma strains with different metastatic potential

作　　者：张宏颖[1] 刘春平[2] 胡春秀[1] 许国旺[1] 

机构地区：[1]中国科学院大连化学物理研究所国家色谱研究分析中心,辽宁大连116023 [2]大连医科大学病理学教研室,辽宁大连116027

出　　处：《中国微生态学杂志》2006年第6期421-423,426,共4页Chinese Journal of Microecology

基　　金：国家自然科学基金资助项目(No.90209048);中国科学院重大方向性项目(KSCX2-SW-329;KGCX2-SW-213)

摘　　要：目的 研究姜黄素（Curcumin,Cur）对来源于同一亲本的小鼠腹水型肝癌高、低淋巴道转移株（HCa-F和HCa-P）增殖、细胞周期分布及皮下移植HCa-F和HCa-P的615小鼠生存期的影响,并探讨2株细胞对Cur的敏感性差异是否存在显著性.方法 用15～250 μmol/L Cur分别处理HCa-F和HCa-P 48 h,以MTT法检测细胞的生长活性,以生长曲线评估药物对细胞生长的作用,以流式细胞仪检测药物对细胞周期分布的影响,观察Cur对细胞形态的影响.给615小鼠右腋中线皮下接种HCa-F和HCa-P细胞,通过ig给药的方法,观察Cur对荷瘤鼠生存期的影响.结果 Cur可抑制HCa-F和HCa-P的生长,抑制率分别为7.23%～82.23%和6.04%～61.31%,且呈剂量依赖性;IG0在HCa-F为51.48μmol/L,在HCa-P为90.87μmol/L;在非细胞毒性最大剂量15μmol/L Cur作用下,2株细胞的生长速度均受到抑制,群体倍增时间延长;HCa-F细胞形态受到影响;细胞周期被重新分布,HCa-F被阻滞在S期,HCa-P被阻滞于S期和G2/M期.经ig给予150 mg/kg的Cur可使皮下接种HCa-F和HCa-P的615小鼠生存率提高分别达45.06%和49.68%.结论 Cur可抑制HCa-F和HCa-P的增殖,使细胞周期重新分布,且高转移细胞株HCa-F较低转移细胞株HCa-P对Cur更为敏感Cur可延长荷瘤鼠的生存期.Objectives Murine ascites hepatocarcinoma cell strains with different metastatatic potential （HCa- F and HCa-P） were pretrcated by eurcumin and 615 mice inoculated subcutaneously with HCa-F and HCa-P were treated by intragastric administration of cucurmin, the effects of curcumin on the biological behavior of the two cell strains and the life span of mice were observed, Methods In vitro, HCa-F and HCa-P had been pretreated by curcumin at 15-250 /Lmol/L for 48 h, the growth of HCa-F and HCa-P were studied by the use of MTT assay,the influence of curcumin on cell proliferation and cell cycle were observed by making growth curve and flow cytometery （FCM）, respectively. The effect of curcumin on the morphology of cells was observed. In vivo, HCa-F and HCa P cells were inoculated subcutaneously to right axilla and the effect of curcumin on the life span of mice was studied, Results The growth inhibition rates of curcumin on HCa-F and HCa-P reached 7.23% -82.23% and 6, 04%-61.31%, respectively,in dose-dependent manner and IC50 was 51.48 /Lmol/L in HCa-F and 90, 87μmol/L in HCa-P. Treated by the maximum uncytotoxicity dose of curcumin 15 /Lmol/L for 48 h, the growth of HCa-F and HCa-P was inhibited, the multiplication time of these two cell strains increased and the cell cycle was redistributed with HCa-F being arrested in S phase while HCa-P being arrested in S and G2/M phases. Intragastric administration of curcumin at dose of 150 mg/kg could significantly increase the life span of the mice prolonging to 45.06 % with HCa- F and 49.68 % with HCa-P. Conclusions The growth of HCa-F and HCa-P are inhibited and the cell cycle is redistributed by curcumin, HCa-F is more sensitive to curcumin than HCa-P is. Intragastric administration of curcumin could significantly increase the life span of mice inoculated with HCa-F and HCa-P.

关 键 词：姜黄素 小鼠腹水型肝癌细胞株 抑制作用 细胞周期 生存期 

分 类 号：R286.91[医药卫生—中药学]