健康老年男性雄激素受体基因CAG重复序列多态性研究  

Study of AR gene CAG repeat polymorphism in old men

作　　者：楚新梅[1] 李小鹰[1] 梁春红[1] 叶玲[1] 刘建伟[1] 

机构地区：[1]解放军总医院,北京100853

出　　处：《中华老年心脑血管病杂志》2006年第12期817-819,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：全军十五面上课题(01AM301)

摘　　要：目的研究健康老年男性雄激素受体(AR)基因CAG重复序列多态性。方法使用DNA测序方法对102例健康老年男性AR基因外显子1 NH_2-端转录调节区内CAG重复序列长度进行测定。结果男性AR基因CAG重复数最短为8,最长为35。CAG重复数平均值为22．83±3．97。CAG重复数分布频率最多的为21(17．6%),22 (14．7%),23(15．7%)和24(6．9%),共占54．9%。结论健康老年男性AR基因CAG重复序列呈现多态性,多数集中在21-24。为进一步研究AR基因变异与疾病的关系提供依据。Objective To analyze CAG length polymorphism of androgen receptor（AIR） gene in old men. Methods A study was performed in 102 randomly selected male healthy subjects aged 62 ～ 92 years in Wanshoulu district, Beijing. DNA sequencing of the CAG repeat in the N-terminal domain of the first exon of the AIR gene was analyzed using Biosystems 3730xl DNA Analyzer. Results The AR allele length ranged from 8-35 CAG repeat in 102 old men. The average value of CAG repeat was 22.83 ± 3.97. The high frequencies of CAG repeat were 21（17.6%）, 22（14.7%）, 23（15.7%） and 24（6.9%）,accounting for 54.9% in total. Conclusion Androgen receptor gene CAG length showed polymorphism in old men, most of the CAG length was focused on 21-24. This study offered the basic data on relationship between AR gene and disease.

关 键 词：受体 雄激素 外显子 DNA 多态性 限制性片段长度 

分 类 号：Q987[生物学—遗传学]