塞来昔布预防大鼠乳腺癌发生及其机制  

Chemopreventive effect of celecoxib against DMBA-induced breast cancer and its mechanism

作　　者：康华峰[1] 王西京[1] 刘小旭[1] 代志军[1] 薛锋杰[1] 薛兴欢[1] 

机构地区：[1]西安交通大学医学院第二附属医院肿瘤外科,陕西西安710004

出　　处：《南方医科大学学报》2006年第11期1599-1602,共4页Journal of Southern Medical University

基　　金：陕西省科技计划项目(2003K10-G40)~~

摘　　要：目的观察环氧化酶-2(COX-2)选择性抑制剂塞来昔布对化学致癌剂7,12-二甲基苯蒽(DMBA)化学诱发的大鼠乳腺癌形成的影响。方法DMBA油剂灌胃复制大鼠乳腺癌模型,90只大鼠分为3组:单纯诱癌组作为阴性对照、三苯氧胺组作为阳性对照和塞来昔布组,观察塞来昔布对大鼠乳腺肿瘤发生率和COX-2、VEGF蛋白表达的影响。结果三苯氧胺组(48.15%)和塞来西布组(50.00%)肿瘤发生率明显低于单纯诱癌组(85.71%),差异具有显著性(P=0.003;P=0.004)。塞来昔布组COX-2蛋白表达率(28.57%)低于单纯诱癌组(83.33%)和三苯氧胺组(69.23%),差异具有显著性(P=0.001;P=0.035)。塞来昔布组VEGF蛋白表达率(42.86%)低于单纯诱癌组(79.17%)(P=0.023);和三苯氧胺组(46.15%)无显著性差异(P=0.863)。结论塞来昔布能抑制DMBA诱发的大鼠乳腺癌的发生、发展,下调COX-2和VEGF蛋白表达可能是其机制之一。Objective To evaluate the chemopreventive effect of celecoxib, a specific cyclooxegenease-2 （COX-2） inhibitor, on chemically induced breast cancer of rats and its effect on COX-2 expression. Methods 7, 12-dimethylbenz anthracene （DMBA） was administered intragastrically in SD female rats to establish breast cancer models, which were divided subsequently into control group, tamoxifen group and celecoxib group to receive different treatments accordingly. The occurrence rate of breast cancer was observed and the effect of celecoxib on COX-2 and vascular endothelial growth factor （VEGF） expressions assayed by immunohistochemical SP method. Results The incidence of breast cancer in tamoxifen group （48.15%） and celecoxib group （50.00%） were both significantly lower than that in the control group （85.71%; P=0.003 and P=0.004, respectively）. The positivity rate of COX-2 expression in celecoxib group （28.57%） was significantly lower than those of tamoxifen group （48.15%） and control group （83.33%; P=0.001 and P=0.035, respectively）. The positivity rate of VEGF expression in celecoxib group （42.86%） was significantly lower than that of control group （79.17%,P=0.023）, but comparable with that in tamoxifen group （46.15%, P=0.863）. Conclusion Celecoxib can significantly suppress DMBA-induced breast cancer in female rats possibly through down-regulation of COX-2 and VEGF expressions.

关 键 词：乳腺肿瘤 塞来昔布 二三苯氧胺 环氧化酶-2 血管内皮生长因子 

分 类 号：R737.9[医药卫生—肿瘤]