人SOD基因的克隆与原核表达  

Cloning and Prokaryotic Expression of Human SOD Gene

作　　者：宋凯[1] 宋继梅[2] 

机构地区：[1]徐州生物工程高等职业学校,江苏徐州221006 [2]西北农林科技大学生物工程研究所,陕西杨陵712100

出　　处：《动物医学进展》2006年第12期91-94,共4页Progress In Veterinary Medicine

基　　金：国家"863"项目(2001AA213081)

摘　　要：超氧化物歧化酶(SOD)是机体内超氧自由基的天然清除剂,在抗衰老、抗辐射、抗逆境,消炎、抑制肿瘤和癌症,以及自身免疫疾病的治疗方面有很重要的作用。我国目前主要从血液中提取SOD,有关重组人SOD的研究较少。文章通过RT-PCR方法克隆得到人SOD基因,并成功构建其原核表达载体pET-16b-SOD,为通过基因重组技术生产人SOD创造了条件。SOD is superoxide dismutase, it can clear away excessive O2^-· which is produced during the course of organism supersession. It can prevent various diseases and delay the decrepitude of human. We have accurately cloned human SOD gene fragment by RT-PCR and constructed the pET-16b-SOD vector. The cloning of human SOD gene and the construction of it ＇s expression vector lay a good foundation for further study on their function,biological activity and application.

关 键 词：人超氧化物歧化酶 基因克隆 原核表达 

分 类 号：Q785[生物学—分子生物学]