影响桑子叶不定芽形成的因素  

FACTORS INFLUENCING THE FORMATION OF ADVENTITIOUS BUDS FROM MULBERRY COTYLEDONS

作　　者：王勇[1] 陈爱玉[1] 倪国孚[1] 

机构地区：[1]中国农业科学院蚕业研究所

出　　处：《蚕业科学》1996年第4期208-213,共6页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金

摘　　要：用正交设计法对影响桑子叶不定芽形成的诸因素进行了比较研究。结果得出：（１）诱导培养基采用ＭＳ无机盐加即维生素、６－苄氨基嘌呤３．０ｍｇ／Ｌ、吲跺乙酸０．３ｍｇ／Ｌ、果糖或葡萄糖３０ｇ／Ｌ、水解乳蛋白５００ｍｇ／Ｌ和琼脂粉６ｇ／Ｌ最为适合，不定芽诱导率达到８０％以上；（２）子叶初期培养中，光照以５００～１０００ｌｘ较为适宜；（３）预培养４～６ｄ的桑种子，其子叶不定芽诱导率较其余子叶为高。不定芽经继代培养后能在生根培养基中形成完整根系，移植到土壤后可成苗。The factors influencing the formation of adventitious buds were studied by means oforthogonal design.The results showed that,(1)A medium containing MS mineral salts,8p vitamins,3.0mg/l of 6-benzylaminopurine,0. 3mg/l of indole-3-acetic acid,30g/l offructose or glucose,500mg/l oflactalbumin hydrolysate,solidified with 6g/l of agarpowder was most favorable for inducing adventitous buds from mulberry cotyledons.With this medium,the induction rate reached more thn 80%,(2)During the initial cul-ture of the cotyledons,illumination at an intensity of 500-1000lx is desirable,(3)Cotyle-dons isolated from mulberry seeds that had undergone preculture mediurn and subse-quently rooted well in rooting medium.

关 键 词：桑树 子叶 不定芽 诱导 

分 类 号：S888.3[农业科学—特种经济动物饲养]