NF-κB抑制剂对于人卵巢癌裸鼠移植瘤及其血管生成的影响  

Effects of NF-κB inhibitor on the growth and angiogenesis of human ovarian cancer xenografts in nude mice

作　　者：何凯[1] 崔满华[1] 王琨[2] 江元[3] 

机构地区：[1]吉林大学第二医院妇产科,吉林长春130021 [2]南方医科大学附属深圳妇幼保健院 [3]宁波市妇女儿童医院

出　　处：《中国妇幼保健》2006年第23期3292-3294,共3页Maternal and Child Health Care of China

摘　　要：目的:初步探讨IκBα磷酸化抑制剂BAY 11-7082对人卵巢癌裸鼠移植瘤生长及血管生成的影响。方法:选用人卵巢癌细胞系COC 1建立裸鼠皮下移植瘤模型,腹腔注射BAY 11-7082,测量移植瘤的重量、体积,并用TUNEL法检测移植瘤细胞凋亡情况。同时应用免疫组化染色,检测瘤组织内的NF-κB p 65、血管内皮生长因子(VEGF)和FⅧ蛋白表达,并计数微血管密度(MVD)。结果:BAY 11-7082组移植瘤重量显著低于阴性对照组(P<0.05),抑制率为47.60%;BAY 11-7082组AI值高于对照组(P<0.05);PI值小于对照组(P<0.05),BAY 11-7082组NF-κB p 65、VEGF与MVD均显著低于对照组(P<0.05)。结论:BAY 11-7082能明显抑制人卵巢癌细胞系COC 1裸鼠移植瘤的生长,通过影响VEGF表达对抗化疗药物。Objective： To study the effect of BAY 11 - 7 082 （ IκBα inhibitor）, on the growth and angiogenesis of human ovarian cancer xenografts in nude mice. Methods： COC1 ceils were selected and were injected subcutaneously in thymus nude mice to establish xenograft models. The mice were divided into control group and BAY 11 -7 082 group （n = 6 in each group） . The inhibitory rate was calculated according to the weights of xenografts. And apoptosis rate of xenografts cells was ＆retted by TUNEL assay. The proteins expressions of NF -κB p 65, vascular endothelial growth factor （VEGF） and F Ⅷ were assessed by immunohistology method, and MVD was counted according to F Ⅷ staining. Results： The weight of xenografts was significantly lower in PDTC group than that in control group （P 〈 0. 05） and the inhibitory rate was 47. 60%. AI in PDTC group was higher than that in the control （P 〈 0. 05）, whereas PI was higher in PDTC group than in the e/milo （P 〈0. 05） . The expressions of VEGF and MVD count of were significantly decreased in PDTC group than that in control group （P 〈0. 05） . Conclusion： BAY 11 -7 082 can inhibit the growth of human ovarian cancer COC1 xenografts in nude mice, which may be due to the down regulation of VEGF thus inhibit tumor angiogenesis.

关 键 词：NF-ΚB 卵巢癌 移植瘤 血管生成 

分 类 号：R737.31[医药卫生—肿瘤]