PCR-DGGE在制浆造纸废水处理微生物检测中的应用  

Application of PCR-DGGE in Microbial Ecosystem Analyses in Pulping and Papermaking Effluent Trentment

作　　者：陈竹[1] 陈元彩[1] 

机构地区：[1]华南理工大学资源科学与造纸工程学院,广州510640

出　　处：《造纸科学与技术》2006年第6期128-131,共4页Paper Science & Technology

基　　金：国家自然科学基金(20676045);华南理工大学制浆造纸工程国家重点实验室开放基金;江南大学工业生物技术教育部重点实验室开放课题(KLIB-KF200503)

摘　　要：聚合酶链式反应结合变性梯度凝胶电泳(PCR-DGGE)技术是近年来微生物分子生态学的研究重点,具有简便快速、可靠性强、不需要进行微生物的培养等优点,被广泛应用于废水处理系统中微生物的多样性和动态性的分析。本文概述了PCR-DGGE技术的发展、原理、影响因素以及在国内外废水处理的微生物检测中的应用现状。将该技术应用于制浆造纸废水处理的微生物检测中对理解污染物的降解机理有非常重要的意义,具有良好的发展前景。At present, PCR - DGGE is the core of the study on microbial molecular ecology. It has been widely used in the analysis of the diversity and dynamics in the waste water treatment process, because it is simple, convenient, swift and credible, and it does not need to be cultivated. The development, the principle and the influence factors of PCR - DGGE are reviewed in this paper. The application of microbial ecosystem analysis in waste water treatment process at home and abroad is also introduced, if this method is used in the waste water treatment of pulping and papermaking, it will be helpful to understand the function of the microbial on the degradation of pollution matter. It will also have a good development prospect.

关 键 词：PCR—DGGE 制浆造纸废水 微生物多样性 

分 类 号：X793[环境科学与工程—环境工程]