重组葡激酶对重症急性胰腺炎大鼠心肌损伤的治疗作用  

Effect of recombinant staphylokinase on inhibiting rats' myocardial damage following severe acute pancreatitis

作　　者：沙建平[1] 陈炫[2] 祝彼得[3] 邹全明[1] 张新胜[4] 陈卫银[3] 赵艳[3] 冯本华[3] 胡蓉[3] 陈永峰[3] 

机构地区：[1]第三军医大学临床微生物学及免疫学教研室,重庆400038 [2]四川大学华西医学中心第一临床医院感染科,四川成都610042 [3]成都中医药大学,四川成都610075 [4]四川大学华西基础医学与法医学院组织胚胎学与神经生物学教研室,四川成都610041

出　　处：《中国中西医结合消化杂志》2006年第6期355-358,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金："十五"863生物领域高技术首批重点课题(2001AA215-161)

摘　　要：[目的]研究重组葡激酶(r-Sak)对大鼠重症急性胰腺炎(SAP)的发展及心肌损伤的干预作用,探讨其作用机制。[方法]63只SD大鼠随机分为假手术(A)组(n=9),模型对照(B)组(n=27),r-Sak治疗(C)组(n=27)。SAP模型采用5%牛磺胆酸钠胰胆管逆行注射方法建立。ELISA法测定6、12、18 h各时点血清肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、肌酸激酶同工酶(CK-MB)及心肌肌钙蛋白(CTn1)水平,免疫组化法检测术后6、121、8 h各时点心肌核转录因子-κB(NF-κB)的活化情况,光镜及电镜下观察胰腺及心肌组织的病理变化。[结果]大鼠从术后6 h开始出现CTn1、CK-MB升高,治疗组各时点较对照组明显下降(P<0.05)。IL-6、TNF-α水平亦从术后6 h开始升高,其变化在时点上与CTn1、CK-MB的变化具有一致性,治疗组各时点与对照组相比明显下降(P<0.05)。治疗组胰腺及心肌组织的出血坏死减轻,微血管血栓的形成减少。[结论]r-Sak能减轻SAP的病变程度和心肌损伤,可能具有治疗SAP及预防心肌损伤等并发症的作用,特异性溶栓剂的应用可能成为治疗SAP的新途径。[Objective]To investigate the effect of recombinant staphylokinase（r Sak）on severe acute pancreatitis （SAP） and severe acute pancreatitis associated myocardial damage. [Methods]： Sixty three male SD rats were divided randomly into the sham-operated group （group A,n=9）, the control group （group B, n=27） and the r-Sak treatment group （group C, n=27）. SAP wasinduced by intraductal administration 5% sodium taurocholate. Artery blood of group A, B and C was obtained at 6h, 12h and 18h after induction of the model. Serum contents of TNFα, IL-6 and CK-MB, CTnl were detected with ELISA. The activity of NF-κB in myocardial tissue was determined by SP immunohistochemical technique. Pancreatic and myocardial tissues were examined under light and electron microscopy. [Result] ： The contents of CTn1, CK-MB in serum was significantly increased in 6 hours after induction of SAP, and the serum levels of TNF- α and IL- 6 were also elevated at the same time. The contents of the CTnl, CK-MB and TNF- α, IL-6 in serum were significantly decreased, compared with those of the control group （P〈0. 05）. The pathological changes of pancreatic and myocardial tissues in the treatment group were alleviated in contrast to the control group. [Conelusion]： R-Sak can lighten the pathological changes of SAP rats, and alleviate myocardial damage following SAP. R-Sak is a promising agent for SAP therapy and the application of thrombolytic agents would be a new strategy for SAP therapy.

关 键 词：重症急性胰腺炎 溶栓剂 葡激酶 心肌损伤 

分 类 号：R576[医药卫生—消化系统]