多囊蛋白-1在肾透明细胞癌及常染色体显性多囊肾病患者体液中含量的比较研究  

Comparative Study on Polycystin-1 in the Body Fluid of the Patients of Clear Cell Renal Carcinoma and Autosomal Dominant Polycystic Didney Disease

作　　者：吴俊[1] 梅长林[1] 赵海丹[1] 戴兵[1] 刘亚伟[1] 

机构地区：[1]第二军医大学附属长征医院肾内科,解放军肾脏病研究所,上海200003

出　　处：《中国中西医结合肾病杂志》2006年第12期711-714,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：国家"十五"重大科技专项项目(No.2002AA2Z3130);国家自然科学基金资助项目(No.30170901;30271523);上海市卫生系统百名跨世纪优秀学科带头人培养基金资助项目(No.97BR047);上海市科委重大基础研究项目(No.02JC14029)

摘　　要：目的比较多囊蛋白-1在肾透明细胞癌、常染色体显性遗传多囊肾病患者及正常人体液中的含量。方法随机选择在本院就诊的肾透明细胞癌患者20例、常染色体显性多囊肾病患者20例及正常健康查体者15例,留取血液及尿液标本各1份。以本实验室制备的鼠源抗多囊蛋白-1单克隆抗体为捕获抗体,以兔源抗多囊蛋白-1多克隆抗体为检测抗体,采用夹心酶联免疫吸附试验(ELISA)定量检测体液中多囊蛋白-1的含量。结果肾透明细胞癌患者血液中多囊蛋白-1含量为(53.613±8.697)μg/L,显著高于多囊肾病患者血液中含量(6.745±3.215)μg/L(P<0.01)及正常人血液中含量(20.386±14.234)ng/ml(P<0.01)。肾透明细胞癌患者尿液中多囊蛋白-1含量为(10.228±7.989)μg/L,显著低于多囊肾病患者尿液中含量(48.268±16.452)ng/ml(P<0.01),与正常人尿液中含量(10.083±8.941)μg/L比较无统计学差异。结论多囊蛋白-1属于一个新的跨膜蛋白家族,在肾透明细胞癌患者血液中表达增加,而在多囊肾患者血液中表达减少,为进一步揭示多囊蛋白-1的功能提供实验室依据。Objective： To comparatively study on polycystin- 1 in the body fluid of the patients of clear cell renal carcinoma and autosomal dominant polyeystie kidney disease. Methods：20 cases of clear cell renal carcinoma, 20 ease of ADPKD and 15 cases of normal control were observed in this study. Every patient collected one blood sample and one urine sample respectively, We established a .sandwich ELISA method, The polycystin - 1 in the serum and urine of clear cell renal carcino ma patients, ADPKD patients and the normal controls were detected. Results： The quantitative analysis revealed that the polycystin-1 in the serum of clear cell renal carcinoma, which was （53.613 ± 8. 697）μg/L, was markedly higher than that in ADPKD and the normal control, which were （6. 745 ±3. 215）μg/L and （20. 386±14. 234 ）μg/L respectively. （P〈0.01） The polycystin- 1 in the urine of clear cell renal carcinoma, which was （10. 228 ± 7. 989）μg/L, was markedly lower than that in ADPKD, which was （48. 268 ± 16. 452）μg/L（P 〈 0.01）. Conclusion： The polycystin- 1 was markedly higher in the serum of the patients of clear cell renal carcinoma, compared with that in ADPKD and normal controls. Data of this study may provide, at least partly, valuable experimental evidence of the function of polycycstin - 1.

关 键 词：常染色体显性多囊肾病 多囊蛋白-1 肾透明细胞癌 

分 类 号：R737.11[医药卫生—肿瘤]