不同流加发酵方式对重组枯草芽孢杆菌生产维生素B_2的影响(英文)  

Effects of Different Fed-Batch Processes on Vitamin B_2Production in Recombinant Bacillus Subtilis

作　　者：陈涛[1] 武秋立[1] 李晓静[1] 陈洵[1] 赵学明[1] 

机构地区：[1]天津大学化工学院,天津300072

出　　处：《天津大学学报》2006年第B06期7-12,共6页Journal of Tianjin University(Science and Technology)

基　　金：Supported by National Natural Science Foundation of China(20536040)the National Project of Key Foundamental Research(2003CB716003)the Development Project of Science and Technology of Tianjin(05YFGZGX4500).

摘　　要：在5 L发酵罐中培养重组枯草芽孢杆菌RH33生产核黄素,该重组菌的染色体上含有多个解除了转录调节的核黄素操纵子．由于过高的初糖浓度导致严重的代谢溢流,因此间歇发酵过程只能得到较低的菌体量和产物产量．分别采用了3种不同的流加培养方式：分批流加、恒速流加和葡萄糖限制流加来减弱代谢溢流并提高核黄素的产量．同间歇培养相比,分批流加培养能微弱提高重组菌的核黄素产量,而恒速流加和葡萄糖限制流加均能显著提高菌体量和核黄素产量．对于恒速流加培养,当流加速率为0．48 mL／min时核黄素产量和菌体量可以分别达到9．8g/L和27．3g/L对于葡萄糖限制流加培养,通过人工控制葡萄糖流加速率使培养基中的葡萄糖浓度为10～2 g/L时,重组菌RH33可以达到最高的核黄素产量和菌体量(分别为12．5g/L和34．7g/L)．在葡萄糖限制流加培养中核黄素产率约为0．06 g(核黄素)／g(葡萄糖),高于其他几种培养方式．A recombinant Bacillus subtilis strain RH33 containing multiple copies of deregulated riboflavin operon in its chromosome was cultured in a 5-L fermenter for riboflavin production. Batch cultivation suffers from low cell mass and riboflavin productions because high initial glucose concentration causes severe metabolic overflow. Three fed-batch modes, intermittent, constant rate and glucose-limited, were carried out to reduce overflow and increase riboflavin production in this paper. Compared with batch mode, intermittent fed-batch mode improved riboflavin production slightly, the constant rate and glucose-limited fed-batch modes can make a significant increase in both dry cell mass and riboflavin production. When the feeding rate was 0.48 mL/min, a riboflavin production of 9.8 g/L and dry cell mass of 27.3 g/L were achieved. Furthermore, a maximum riboflavin titcr of 12.5 g/L and dry cell mass of 34.7 g/L were obtained in a glucose-limited fed-batch process by maintaining constant （1-2 g/L） glucose concentration in the culture broth and manually controlling the glucose feed rate. The maximum riboflavin yield was nearly 0.06 g（ riboflavin）/g（glucose） in the glucose-limited mode, which was slightly higher than that of batch mode and other fed-bath modes.

关 键 词：枯草芽孢杆菌 核黄素 间歇培养 流加培养 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]