枸杞多糖对人宫颈癌细胞生长及细胞凋亡影响  

Effect of Lycium barbarum polysaccharides on growth of human cervical carcinoma Hela cell and cell apoptosis

作　　者：崔晓燕[1] 罗琼[1] 杨明亮[1] 王丽红[1] 李丹丹[1] 梁成振[1] 闫俊[1] 李卓能[1] 

机构地区：[1]武汉大学公共卫生学院营养与食品卫生学系,武汉430071

出　　处：《中国公共卫生》2006年第12期1411-1412,共2页Chinese Journal of Public Health

基　　金：湖北省科技厅重点攻关课题(2001AA307B);达能膳食营养研究与宣教基金(200305)

摘　　要：目的观察枸杞多糖对体外培养的人宫颈癌Hela细胞生长及其细胞凋亡的影响。方法不同剂量的枸杞多糖作用于体外培养的宫颈癌Hela细胞,用苔盼蓝染色、四甲基偶氮噻唑蓝(MTT)法及流式细胞仪检测人宫颈癌Hela细胞存活率、抑制率及细胞凋亡等指标。结果枸杞多糖可明显抑制人宫颈癌Hela细胞的生长,抑制率可达96.85%,并呈剂量效应关系,细胞凋亡率可达36.8%。结论枸杞多糖对人宫颈癌Hela细胞生长具有抑制作用,其抑制机制可能是通过影响人宫颈癌细胞的生长周期和诱导其凋亡而实现。Objective To investigate the cell growth and cell apoptosis of Lycium barbarum polysaccharides （LBP） treated on human cevical carcinoma Hela cell cultured in vitro. Methods Hela cells were treated with different doses of LBP, the rate of cell growth was detected by using trypan blue exclusion staining, and taking MTT assay to analyze the inhibition of cell growth, then the rate of cell apoptosis was measured by flow cytometry. Results LBP could increasingly inhibit the growth of cervical carcinoma Hela cell line with dose-effect. The biggest inhibition rate was up to 96.85 % in those treated with LBP, and the apoptosis rate was 36.8 %. Conclusion LBP could inhibit the growth of Hela cell line in vitro, and the mechanism might be related to the influence of LBP in the cell cycle and the cell apoptosis induced by LBP.

关 键 词：枸杞多糖 人宫颈癌HELA细胞 生长抑制率 细胞凋亡 

分 类 号：R151.2[医药卫生—营养与食品卫生学]