发酵莲子乳对小鼠急性CCl_4肝毒性保护作用  

Protective effect of lotus-seed milk fermented product on acute CCl_4 hepatic toxicity in mice

作　　者：吴小南[1] 林建银[2] 陈洁[1] 汪家梨[1] 

机构地区：[1]福建医科大学公共卫生学院,福州350004 [2]福建医科大学分子医学研究中心

出　　处：《中国公共卫生》2006年第12期1488-1490,共3页Chinese Journal of Public Health

基　　金：教育部重点项目基金资助(02074)

摘　　要：目的研究发酵莲子乳(LMFP)对急性四氯化碳(CCl4)肝毒性的保护作用。方法检测LMFP上清液对体外肝匀浆过氧化脂质生成量的影响。干预14 d,一次性腹腔注射0.1%CCl4造模,测定肝脏损伤及抗氧化指标,肝组织切片苏木素-伊红(HE)染色及免疫组化观察。结果LMFP于体外可抑制正常肝匀浆及经半胱氨酸、硫酸亚铁激发肝匀浆脂质过氧化物(LPO)的生成;与模型组比较,LMFP干预组肝细胞肿胀,局灶坏死,炎细胞浸润等病理损伤减轻,Bcl-2表达增强;肝匀浆乳酸脱氢酶(LDH)活力降低,LPO生成显著减少,超氧化物歧化酶(SOD)活性增加(P<0.05)。结论LMFP能够通过抗氧化系统发挥作用,减轻CCl4所致小鼠急性肝毒性。Objective To explore the protective effect and possible mechanism of Lotus- seed Milk Fermented Product （LMFP） from acute CCl4 hepatic toxicity in mice. Methods Ascertain the effect of lotus - seed milk fermented product on LPO of normal liver homogenate and normal hepatic homogenate excitated by eysteine and ferrous sulfate in vitro. Mice were fed with distilled water, 25 %, 50%, 100 % LMFP and VE, intraperitoneal injected Carbon Tetrachloride（CC14） vegetal oil admixture in the 14th day. The examines assessing the degree of the hepatic acute damage and peroxidization were executed. Pathomorphological changes were detected by Haematoxylin eosin （HE） coloration and immunohistoehemistry. Results Lactiperoxidase （LPO）level of normal liver homogenate and hepatic homogenate excitated by cysteine and FeSO4 in LMFP groups were significantly decreased compared with the control group. Compared with the model group, Hepatocyte swell, necrosis and inflammatory cells penetration in the LMFP and VE groups were relieved, Bcl - 2 express enhanced. Latic dehydrogenase （LDH） activity was decreased, LPO level was observably decreased, Superoxide dismutase （SOD） activity was significantly increased in LMFP groups. （ all Significance, P 〈 0.05）. Conclusion LMFP can protect liver from acute CCl4 hepatic toxicity by the mechanism of anti - oxide system.

关 键 词：发酵莲子乳(LMFP) 膳食补充 四氯化碳 过氧化反应 

分 类 号：R151.3[医药卫生—营养与食品卫生学]