利用β-乳球蛋白遗传变异体检测牦牛乳中的普通牛乳  

DETECTION OF BOVINE MILK IN YAK MILK UTILIZING β-LACTOGLOBULIN GENETIC VARIANTS

作　　者：陈琛[1] 胡强[1] 郑玉才[1] 文勇立[1] 陈炜[1] 苏永杰[1] 朴影[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《食品研究与开发》2006年第11期157-159,共3页Food Research and Development

基　　金：四川省应用基础项目(06JY0289);西南民族大学大学生科技创新项目

摘　　要：建立简便、可靠的方法检测牦牛乳中添加的普通牛乳。在牦牛乳中加入5%￣30%的普通牛乳,采用等电点沉淀法制备乳清,然后用聚丙烯酰胺凝胶电泳分离乳清蛋白。根据牦牛与普通牛β-乳球蛋白遗传变异体在电泳中的迁移率差异,可检测到牦牛乳中添加的5%的普通牛乳。该法也适用于牦牛奶粉的检测,对牦牛乳制品的质量监测有实际应用价值。The objective of this research was to develop a simple and reliable approach to detect the addition of bovine milk to yak milk. Bovine milk was added to yak milk at the ratios from 5 % to 30 %, and whey was prepared using isoelectronic point precipitation method. Whey proteins were separated with native polyacrylamide gel electrophoresis. The addition of 5 % of bovine milk to yak milk could be detected based on the mobility differences of β-1actoglobulin genetic variants on the gel. This protocol can also be used in the assay of yak milk powder, and is of importance in the quality control of yak milk products.

关 键 词：牦牛 乳 奶粉 Β-乳球蛋白 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]