苦豆子胶多糖组分气相色谱与薄层色谱分析  

ANALYSIS OF POLYSACCHARIDE COMPOSITION OF SOPHORA ALOPECUROIDES SEED BY GC AND TLC

作　　者：杨永利[1] 郭守军[1] 罗军德[2] 马瑞君[1] 

机构地区：[1]韩山师范学院生物系,广东潮州541041 [2]兰州大学生命科学学院,甘肃兰州730000

出　　处：《食品研究与开发》2006年第11期159-162,共4页Food Research and Development

基　　金：广东省自然科学基金资助(06028879)

摘　　要：利用铜沉和醇沉法提取纯化多糖,以薄层色谱和气相色谱法进行定性和定量分析,建立苦豆子胶多糖组分分析方法。结果苦豆子胶多糖是由甘露糖和半乳糖组成,其百分含量分别为39.9395%和17.8849%;相对标准偏差为3.5602%￣5.0763%,回收率为98.63%￣99.68%;二者配比为半:甘=1:2.23;总糖含量为57.8244%;结论:该方法便捷,可靠,可作为苦豆子胶多糖组分定性和定量的分析方法.To establish an analysis method of saccharide components. The saccharide was extracted with hot water, and purified with Fehling reagent and 95 % ethanol; the saccharide components of sophora alopecuroides gum was analysized by TLC and GC. The polysaccharide of sophora alopecuroides gum was composed of D-galactose and D-mannose at ratio 1 ： 2.23, and their percentage are 17.884 9 % and 39.939 5 %, respectively, with a relative standard deviation of 3.560 2 % ～ 5.076 3 %, and the recovery 98.63 %～99.68 %. The method is reliabale, and can be used for a qualitative and quantitative method of saccharide components of sophora alopecuroides gum.

关 键 词：苦豆子 苦豆子胶 多糖 薄层色谱 气相色谱 

分 类 号：TS201.1[轻工技术与工程—食品科学]