C-myc、P16和Bc1-2蛋白在胆管癌中表达及意义  

Significance of the expression of C-myc,P16,Bcl-2 proteins in hilar cholangiocarcinoma

作　　者：李灼日[1] 吴金术[1] 毛先海[1] 田秉璋[1] 

机构地区：[1]湖南师范大学第一附属医院湖南省人民医院肝胆外科,湖南长沙410005

出　　处：《中国现代医学杂志》2006年第22期3427-3430,共4页China Journal of Modern Medicine

摘　　要：目的检测胆管癌组织中C-myc、P16、Bcl-2蛋白表达,探讨其与胆管癌病理的关系及其在胆管癌发生、发展中的作用。方法用免疫组化法检测胆管癌、癌旁组织、胆管囊状扩张症组织及医源性胆道损伤的胆管组织中C-myc、P16和Bcl-2蛋白表达。结果C-myc、P16在胆管癌组织中的阳性表达率明显高于其他组(P<0.05);C-myc、P16阳性率在高、中分化腺癌中明显高于低分化腺癌(P<0.05);C-myc、P16阳性率在I级胆管癌明显高于II、III级胆管癌(P<0.05);癌旁组织中Bcl-2阳性表达率为100%,明显高于癌组织(P<0.05);Bcl-2阳性率在不同组织学级别中差异无显著意义(P>0.05);无转移组和有转移组组间比较,C-myc阳性表达率差异有显著性(P<0.05),P16和Bcl-2阳性率差异无显著性(P>0.05)。结论C-myc、P16蛋白表达特征可反映胆管癌生物学行为和恶性程度,Bc1-2过度表达与胆管癌变过程有关,可能是胆管癌的早期事件。[Objective] To examine the expression of C-myc, P16, Bcl-2 proteins in hilar cholangioeareinoma （HCC） in order to study their relation with the pathologic characteristic of HCC and their roles in the development of HCC. [Methods] Using immunohistochemical method to detect the expression of c-myc, P16, Bcl-2 proteins in HCC tissues, paracancer tissues, cholagioectasis tissues and normal bile duct tissues. [Results] As to the expression of C-myc and p16, HCC tissue was significantly higher than others （P 〈0.05）, well and medial differentiated HCC tissue obviously higher than poor ones （P 〈0.05）, class 1 HCC significantly higher than class II or III HCC （P 〈0.05）. As to the expression of Bcl-2, paracancer tissue （100%） was significantly higher than other 3 tissues, but differentiation degree of HCC shows no obvious difference; When compared between metastasis and nonmetastasis group （P 〈 0.05）, it is the expression of C-myc other than p16 and Bcl-2 shows a statistic difference （P 〈0.05）. [ Conclusions] The expression of C-myc and P16 proteins probably reflects the biological behavior and malignant degree of HCC, and the over expression of Bcl-2 is related to the development of cholangiocareinoma.

关 键 词：肿瘤相关基因 胆管癌 凋亡 免疫组化 

分 类 号：R735.8[医药卫生—肿瘤]