日本百脉根■牛儿基■牛儿基氢化酶的电子克隆及进化分析  

In Silico Cloning and Evolution Analysis of Geranylgeranyl Hydrogenase Gene from Lotus corniculatus var.Japonicus

作　　者：李晓晓[1] 李蕊[1] 李雅轩[1] 蔡民华[1] 胡英考[1] 

机构地区：[1]首都师范大学生命科学学院,北京100037

出　　处：《安徽农业科学》2006年第23期6145-6147,6150,共4页Journal of Anhui Agricultural Sciences

基　　金：北京市教委科技发展计划项目基金(200310028112)资助

摘　　要：根据物种间同源基因相对保守的特点,利用生物信息学的方法,以拟南芥牛儿基牛儿基氢化酶(GGH)基因cDNA序列为信息探针,搜索GenBank中的HTGs数据库,找到一个与之高度匹配的日本百脉根基因组DNA序列LJT46M09,用GENSCAN软件分析该序列得到一个包含2个外显子和1个内含子的基因。以此基因序列BLAST检索GenBank中的est-others数据库获得了同源的EST片段。进行EST拼接后得到该基因的cDNA序列,GenBank登录号为DQ013361,由1 389 bp核苷酸组成,具有完整的开放阅读框架(ORF),推测编码蛋白为462个氨基酸。推导的氨基酸序列与大豆、截形苜蓿、烟草、拟南芥、小麦、水稻的牛儿基牛儿基氢化酶基因氨基酸序列的一致率分别为91%、89%、84%、81%7、3%、74%。该基因与叶绿素等的生物合成有关。With the development of EST projects and bio-informatics, in silico cloning has been a new strategy of gene cloning. With Arabidopsis thaliana GGH cDNA sequence as a querying probe to search the Lotus corniculatus vat. japonicus HTGs database, a highly homologous DNA sequence, which was referred as LjT46M09 clone, was obtained, The result of gene prediction with performing GENSCAN program showed that this gene contained 2 exons and 1 intron. With this predicted sequence to BLAST GenBank est-others database, many homologous EST sequences, which can be edited a unique sequence, was obtained, which was the same as the predicted sequence from genomie DNA. The full-length genc of 1 389 bp contained a complete ORF, encoding 462 amino acids. Its GenBank accession number was DQ013361. The deduced amino acid showed 91%, 89%, 84%, 81%, 73% and 74% homology with GGH from Glycine max, Medieago truncatula, Nicotiana tabacum, Arabidopsis thaliana and Triticum aestivum, respectively. This eDNA sequence was confirmed with EST sequences.

关 键 词：日本百脉根 牻牛儿基牻牛儿基氢化酶(GGH) 电子克隆 EST 

分 类 号：Q55[生物学—生物化学]