K562细胞外泌体诱导特异性CTL生成的研究  

Production of Specific CTL Induced by Exosomes Derived from K562 Cells

作　　者：陈绍倩[1] 杜英[2] 王鑫[3] 顾巧丽[3] 黄玉敏[2] 董子明[3] 

机构地区：[1]郑州大学第一附属医院血液科,郑州450052 [2]郑州大学基础医学院免疫教研室,郑州450052 [3]郑州大学基础医学院病理生理教研室,郑州450052

出　　处：《中国实验血液学杂志》2006年第6期1168-1171,共4页Journal of Experimental Hematology

摘　　要：为了研究人白血病细胞株K562细胞分泌的外泌体(exosomes)及K562细胞总RNA刺激人树突状细胞(DC)分泌的外泌体能否诱导出K562细胞特异性CTL,采用四步离心法提取K562细胞及K562细胞总RNA刺激人树突状细胞的培养上清液中的外泌体,并诱导CTL生成,以四甲基偶氮唑蓝(MTT)法检测CTL对K562细胞的杀伤作用。结果显示K562细胞外泌体和K562细胞RNA刺激的DC和外泌体均能明显促进对K562细胞的杀伤活性,与未经外泌体作用的对照组相比有显著性差异(P<0.05)。外泌体作用后对K562细胞的杀伤作用明显比对HL-60细胞的杀伤作用强,其差异有显著性(P<0.05)。结论K562细胞外泌体能够诱导出特异性抗白血病细胞的免疫反应。The aim of this study was to investigate whether exosomes derived from K562 cells and human monocyte-derived dendritic cells （DCs） transfected with total RNA of K562 cells are capable of inducing antigen-specific cytotoxic T lymphocytes （CTL） responses in vitro. DCs were generated from peripheral blood mononuclear cells （PBMNC） of healthy volunteers in the presence of GM-CSF and IL-4, and then were transfected with K562 RNA by using DOTAP lipofection. Exosomes was extracted from the supernatant of DCs and K562 cells. The T cell were activated to be tumor specific CTL after DCs and exosomes were co-cultured with autologous T cells derived from healthy volunteers＇ PBMNC. The effect of CTL on K562 cells was detected by MTT assay. The results showed that treatment of T cells with exosomes derived from K562 cells or DCs transfected with total RNA of K562 cells could significantly promote their killing ability on K562 cells as compared with untreated T cells （ P 〈 0.05 ）. The killing ability of T cells treated with exosomes on K562 cells was stronger than on HL-60 cells （ P 〈 0.05 ）. It is concluded that the specific CTL immune response to leukemia cells can be induced by exosomes derived from K562 cells.

关 键 词：K562细胞 树突状细胞 外泌体 细胞毒T淋巴细胞 

分 类 号：R733.7[医药卫生—肿瘤] R730.51[医药卫生—临床医学]