兔出血性视网膜脱离感光细胞凋亡的研究  

Cellular apoptosis in hemorrhage retinal detachment in rabbits

作　　者：刘玮[1] 肖航[2] 惠延年[3] 王雨生[3] 张自峰[3] 

机构地区：[1]第三军医大学大坪医院野战外科研究所眼科,重庆400042 [2]第四军医大学西南医院老年病科,重庆400038 [3]第四军医大学西京医院眼科.全军眼科研究所,西安710032

出　　处：《重庆医学》2006年第23期2138-2141,共4页Chongqing medicine

基　　金：全军医药卫生科研基金课题资助(01MA189)

摘　　要：目的观察出血性视网膜脱离（HRD）后视网膜细胞的凋亡情况和凋亡相关基因p53在其中的表达，探讨凋亡在出血性视网膜脱离中的作用。方法21只青紫蓝兔的42只眼，随机平均分为2组，实验组经玻璃体腔向视网膜下注入自体抗凝血0.2ml，对照组视网膜下注入含肝素钠生理盐水0．2ml。2只青紫蓝兔的4只眼作为正常对照组。分别在手术后1h和1．3、7、10、14、28d对视网膜进行细胞凋亡检测实验，并采用免疫组化染色和原位杂交检测方法观察P53在视网膜中的表达情况。结果HRD后1d视网膜外核层即出现大量凋亡细胞，Sd达到峰值，7d内核层出现凋亡细胞。HRD后1～7d的p53表达显著，其中在Sd达到峰值·实验组1、3、7d的p53阳性细胞较对照组明显增多（P＜0．001）。结论 凋亡可能是HRD视网膜损伤的重要机制。Objective To observe the cellular apoptosis of retina and the expression of p53 that is an apoptosis-related gene in a rabbit model of hemorrhage retinal detaehment（HRD）. To ewduatc the effect of apoptosis in HRD. Methods Forty-two eyes of 21 rahbits were divided into two groups randomly; in the experimental group, HRD was established with subretinal autologous blood 0.2ml;and in the control group ,the heparine sodium was injected eorrcspondingly. Four eyes of 2 rabbits were the normal control group. Apoptotic cells were detected by TUNEL assay in 1h.1d.3d.7d,10d.14d,28d after the establishment of H RD. The expressions of p53 gene protein and mRNA in the, models of HRI） and RD were evaluated with immunohistoehemistry and in situ hybridization. Results There were many TUNEI,-positive cells in the outer retinal layers ld after the establishment of HRD models ,and the number of TUNEL positive cells reached a peak 3d after HRD. Then apoptotic cells appeared in the inner retinal layers 7d after HRD. There were significant expressions of p53 ld to 7d after HRD and the positive cells peaked in 3d. In 1d,3d and 7d,the num bcr of positive cells in experimental group were higher than that in the control group（P〈0. 001）. Conclusion Apoptosis may play an important role in mechanism of HRD.

关 键 词：视网膜脱离 眼出血 凋亡 P53 疾病模型 动物 

分 类 号：R774.1[医药卫生—眼科]