禽源致病性沙门氏菌四环素耐药性基因tetC的PCR扩增及序列分析  

PCR amplification and nucleotide sequences analysis of tetracycline resistance gene(tetC) of avian pathogenic salmonell

作　　者：张正庆[1] 罗薇[1] 刘亚刚[1] 于学辉[1] 傅安静[1] 黄兴[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《畜禽业》2006年第12期18-20,共3页Livestock and Poultry Industry

摘　　要：通过对临床分离并经生化试验和血清学试验鉴定的20株禽源沙门氏菌和1株标准株C79-13进行药敏试验,结果证明:有19株有高耐药性,另外2株有低耐药性,耐药率达100%;对其四环素耐药基因tetC进行了PCR扩增,结果获得以质粒为模板的特异性产物,与药敏试验符合率为100%;利用PCR检测四环素耐药基因tetC具有很高的敏感性和特异性,从而为禽类致病性沙门氏菌的耐药性检测提供了高效敏感的手段.By using clinical isolates and biochemical and serologic tests deteation on 20 avian salmonella strains and one standard sahnoaella, the drugsnsceptihility test shows that： 19 strains are with high resistance,another 2 is low. The drug-susceptibility testa also shows that the rate is 100%. After amplifying the tetracycline-resistance gone, it produces specific material by plasmids complete and the concordance rate to drug-susceptibility test is 100%.The result indicates that the methods of PCR to detect tetracycline-resistant gene has the character of susceptibility and speciality.The paper will provides effective and exact methods to detect the drug-resistance of avian salmoaclla.

关 键 词：沙门氏菌 四环素耐药基因 TETC PCR 核酸探针 

分 类 号：S852.61[农业科学—基础兽医学]