WNK4基因G1816T多态性与高血压的关联研究  

Association analysis between G1816T polymorphism of WNK4 gene and essential hypertension.

作　　者：孙志军[1] 李妍[2] 吕晶玉[2] 时景璞[3] 李岭[2] 赵彦艳[2] 

机构地区：[1]中国医科大学附属盛京医院心内科,辽宁沈阳110004 [2]中国医科大学医学遗传学教研室,辽宁沈阳110001 [3]中国医科大学流行病学教研室,辽宁沈阳110001

出　　处：《中国实用内科杂志：临床前沿版》2006年第12期1974-1976,共3页

基　　金：国家自然科学基金资助(30300204)

摘　　要：目的阐明WNK4基因G1816T多态性与原发性高血压的关系。方法2001年3～10月选择中国东北高血压病高发地区辽宁省彰武县采集的259例原发性高血压患者和235名健康对照组的血样应用聚合酶链反应-限制性长度片段长度多态性分析（PCR—RFLP）方法检测G1816T多态，结果经测序验证，同时检测所有人群的血糖、血脂等生化指标。结果原发性高血压组WNK4基因G1816T多态T等位基因频率显著高于对照组（25．9％对20．2％，P=0．035）；AS和SS基因型与收缩压及舒张压升高密切相关（P分别为0．041和0．032）。结论WNK4基因G1816T多态与高血压密切相关。Objective To elucidate the association of WNK4 gene G1816T polymorphism with essential hypertension. Methods Genotyping was performed using polymerase chain reaction -restriction fragment length polymorphism （ PCR - RFLP） and confirmed by direct sequencing. 259 unrelated patients with essential hypertension and 235 unrelated normal controls were recruited. All the subjects had routine laboratory tests. Results The frequency of the T allele of exon8 G1816T polymorphism in hypertension group is significantly higher than that in controls （25. 9% versus 20. 2%, P = 0. 035 ）. The AS and SS genotypes were closely associated with raised systolic and diastolic blood pressure （ P =0. 041, 0. 032 respectively）. Conclusion The WNK4 gene G1816T polymorphism is associated with essential hypertension.

关 键 词：原发性高血压 WNK4基因 单核苷酸多态 

分 类 号：R5[医药卫生—内科学]